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The Effect of Wnt Family Member 5a Gene Silencing on the Proliferation of Achondroplasia Using a DNAzymes-CoOOH Nanocomposite
- Source :
- Journal of biomedical nanotechnology. 17(7)
- Publication Year :
- 2021
-
Abstract
- Achondroplasia is a kind of congenital dysplasia due to the defect of endochondral ossification. Achondroplasia is considered to be a protein folding disease leading to endoplasmic reticulum stress. Endoplasmic reticulum stress may lead to disease by affecting the function and survival state of chondrocytes, but the specific mechanism requires further study. In this study, bioinformatics methods, online database mining, screening of differentially expressed genes for pathway enrichment, and interaction analysis were conducted to detect the Wnt family member 5a (Wnt5a) gene. Additionally, we designed a novel DNAzymes-based nanocomposite that can simultaneously silence Wnt5a genes in chondrocytes. The nanocomposite was composed of amino-functionalized cobalt oxyhydroxide nanoflakes modified by DNAzymes that target the Wnt5a gene. Further, we conducted in vitro experiments to verify that Wnt5a can mediate the mitogen-activated protein kinase signaling pathway through the endoplasmic reticulum stress pathway to affect the proliferation of chondrocytes.
- Subjects :
- Chemistry
Endoplasmic reticulum
Biomedical Engineering
Wnt signaling pathway
Pharmaceutical Science
Medicine (miscellaneous)
Bioengineering
Oxides
Cobalt
DNA, Catalytic
Wnt-5a Protein
Cell biology
Achondroplasia
Nanocomposites
WNT5A
Gene silencing
Humans
General Materials Science
Family
Gene Silencing
Signal transduction
Protein kinase A
Gene
Endochondral ossification
Cell Proliferation
Subjects
Details
- ISSN :
- 15507033
- Volume :
- 17
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of biomedical nanotechnology
- Accession number :
- edsair.doi.dedup.....da777bcd1e2353190c5ceed5893448d7