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Monitoring for adsorption of human serum albumin and bovine serum albumin onto bare and polystyrene-modified silver electrodes by quartz crystal impedance analysis
- Source :
- Journal of biotechnology. 89(1)
- Publication Year :
- 2001
-
Abstract
- The adsorption of human serum albumin (HSA) and bovine serum albumin (BSA) from PBS (pH 7.4) onto bare and polystyrene (PS)-modified silver electrodes was in situ monitored using quartz crystal impedance analysis. The adsorption characteristics of HSA and BSA were discussed by analyzing piezoelectric parameter simultaneous responses. Experimental results indicated that for both HSA and BSA, the amount adsorbed on bare silver was more than that on PS-modified surface. The BSA amount adsorbed on the two surfaces was more than that of adsorbed HSA. A kinetic model was developed to describe the adsorption process and fitted to the experimental data of frequency shift. It was shown that HSA adsorption could be described by a kinetic equation involving two consecutive reactions. At lower concentration, BSA adsorption only involved the first reaction. At higher concentration, BSA adsorption on PS-modified surface involved two consecutive reactions. All fitted results were well in agreement with the corresponding experimental results. The regression values of reaction rate constants for the HSA and BSA adsorption were obtained. These data exhibited difference in adsorption kinetics under different conditions.
- Subjects :
- Silver
Surface Properties
Inorganic chemistry
Serum albumin
Bioengineering
Applied Microbiology and Biotechnology
Reaction rate
Crystal
chemistry.chemical_compound
Adsorption
medicine
Animals
Humans
Bovine serum albumin
Electrodes
Serum Albumin
Chromatography
biology
Chemistry
Chemical modification
Serum Albumin, Bovine
General Medicine
Quartz
Human serum albumin
body regions
Kinetics
embryonic structures
biology.protein
Polystyrenes
Cattle
Polystyrene
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 01681656
- Volume :
- 89
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of biotechnology
- Accession number :
- edsair.doi.dedup.....da7f70d8da504ebf88edc46bc9ffd75f