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Cisplatin induces loop structures and condensation of single DNA molecules
- Source :
- Nucleic Acids Research
- Publication Year :
- 2009
- Publisher :
- Oxford University Press, 2009.
-
Abstract
- Structural properties of single lambda DNA treated with anti-cancer drug cisplatin were studied with magnetic tweezers and AFM. Under the effect of low-concentration cisplatin, the DNA became more flexible, with the persistence length decreased significantly from approximately 52 to 15 nm. At a high drug concentration, a DNA condensation phenomenon was observed. Based on experimental results from both single-molecule and AFM studies, we propose a model to explain this kind of DNA condensation by cisplatin: first, di-adducts induce local distortions of DNA. Next, micro-loops of approximately 20 nm appear through distant crosslinks. Then, large aggregates are formed through further crosslinks. Finally, DNA is condensed into a compact globule. Experiments with Pt(dach)Cl(2) indicate that oxaliplatin may modify the DNA structures in the same way as cisplatin. The observed loop structure formation of DNA may be an important feature of the effect of platinum anti-cancer drugs that are analogous to cisplatin in structure.
- Subjects :
- Magnetic tweezers
Organoplatinum Compounds
Antineoplastic Agents
Biology
DNA condensation
Microscopy, Atomic Force
chemistry.chemical_compound
Genetics
medicine
A-DNA
Molecular Biology
Persistence length
Cisplatin
DNA
Bacteriophage lambda
Elasticity
Oxaliplatin
chemistry
DNA, Viral
Biophysics
DNA supercoil
Nucleic Acid Conformation
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 13624962 and 03051048
- Volume :
- 37
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....da920d5ea381fc7db4d1acab0d679222