Back to Search
Start Over
Hypoglycemic effects of esculeoside A are mediated via activation of AMPK and upregulation of IRS-1
- Source :
- BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-9 (2019), BMC Complementary and Alternative Medicine
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Background Tomato fruit (Lycopersicon esculentum Mill.) has been suggested to be useful for the prevention of diabetes. Esculeoside A is the main saponin compounds in tomatoes. This study investigated the hypoglycemic effects and the underlying mechanism of esculeoside A in C57BLKS/Leprdb (db/db) mice. Methods Wild-type C57BLKS (db/dm) mice were used in the db/dm mouse group and db/db mice were randomly divided into 2 groups: untreated and treated db/db mouse groups. Esculeoside A (100 mg/kg) was administered by gavage for 56 days to the treated db/db mouse group. Distilled water was administered to the db/dm mouse group and the untreated db/db mouse group. The blood and liver biochemical parameters and the expression of liver insulin signaling-related proteins were examined. Results The results showed that esculeoside A reduced the fasting blood glucose (FBG) levels and improved the glucose tolerance. Further investigation revealed that hepatic protein expressions of total AMP-activated protein kinase (T-AMPK), phosphorylated AMP-activated protein kinase (p-AMPK), insulin receptor substrate-1 (IRS-1), and glucokinase (GCK) were significantly upregulated after esculeoside A treatment. In contrast, the hepatic protein expression of phosphoenolpyruvate carboxykinase (PEPCK) was significantly downregulated by esculeoside A treatment. Conclusion These findings suggested that esculeoside A has a potential of alleviating the metabolic abnormalities in db/db mice via regulation of AMPK/IRS-1 pathway. Our findings supported a possible application of esculeoside A as a functional supplement for diabetes treatment.
- Subjects :
- Male
AMPK
medicine.medical_specialty
Sapogenins
medicine.medical_treatment
AMP-Activated Protein Kinases
db/db mice
Liver glucose metabolism
Mice
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Internal medicine
Diabetes mellitus
Glucokinase
medicine
Animals
Humans
Hypoglycemic Agents
Insulin
Phosphorylation
IRS-1
Protein kinase A
Esculeoside a
biology
Chemistry
lcsh:Other systems of medicine
General Medicine
lcsh:RZ201-999
medicine.disease
Up-Regulation
030205 complementary & alternative medicine
Mice, Inbred C57BL
Insulin receptor
Endocrinology
Diabetes Mellitus, Type 2
Complementary and alternative medicine
030220 oncology & carcinogenesis
Insulin Receptor Substrate Proteins
biology.protein
Phosphoenolpyruvate carboxykinase
Research Article
Subjects
Details
- ISSN :
- 14726882
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- BMC Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....dac9cf864771cc351792fc11865d50a2