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Human neutrophils interact with both 6-sulfo LacNAc+ DC and NK cells to amplify NK-derived IFNgamma: role of CD18, ICAM-1 and ICAM-3
- Publication Year :
- 2011
-
Abstract
- The role of neutrophils as key players in the regulation of innate and adaptive immune responses is increasingly being recognized. We report that human neutrophils establish a network with both natural killer (NK) cells and 6-sulfo LacNAc+ dendritic cells (slanDCs), which ultimately serves to up-regulate NK-derived interferonγ (IFNγ). This network involves direct reciprocal interactions and positive amplification loops mediated by cell-derived cytokines. Accordingly, we show that after lipopolysaccharide + interleukin-2 (IL-2) or IL-15/IL-18 stimulation, neutrophils directly interact with and potentiate the activity of both slanDCs and NK cells. On the one hand, neutrophils augment the release of IL-12p70 by slanDCs via a CD18/ intercellular adhesion molecule-1 (ICAM-1) interaction that stimulates activated NK cells to produce IFNγ. IFNγ further potentiates the interaction between neutrophils and slanDCs and the release of slanDC-derived IL-12p70, thus creating a positive feedback loop. On the other hand, neutrophils directly costimulate NK cells via CD18/ICAM-3, leading to the production of IFNγ. Colocalization of neutrophils, NK cells, and slanDCs, as well as of IL-12p70 and IFNγ, in inflamed tissues of Crohn disease and psoriasis provides strong evidence for a novel cellular and cytokine cooperation within the innate immune system in which neutrophils act as amplifiers of NK cell/slanDC-mediated responses.
- Subjects :
- Human neutrophils
6-sulfo LacNAc+ DC
NK-derived IFNgamma
role of CD18
ICAM-1
ICAM-3
Lipopolysaccharide
Neutrophils
medicine.medical_treatment
Immunology
Intercellular Adhesion Molecule-1
Biology
Biochemistry
chemistry.chemical_compound
Interferon-gamma
Immune system
Antigen
Crohn Disease
Antigens, CD
medicine
Humans
Psoriasis
NK-derived IFN{gamma}
Cells, Cultured
Inflammation
Interleukin-15
Innate immune system
Interleukin-18
Amino Sugars
Cell Biology
Hematology
Dendritic Cells
Cell biology
Killer Cells, Natural
Cytokine
chemistry
Interleukin 12
Interleukin-2
Cell Adhesion Molecules
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....dada5af81d04e7d5ccca1728c9b1387b