Back to Search
Start Over
Comparison of widely used Listeria monocytogenes strains EGD, 10403S, and EGD-e highlights genomic differences underlying variations in pathogenicity
- Source :
- Bécavin, C; Bouchier, C; Lechat, P; Archambaud, C; Creno, S; Gouin, E; et al.(2014). Comparison of widely used Listeria monocytogenes strains EGD, 10403S, and EGD-e highlights genomic differences underlying variations in pathogenicity. mBio, 5(2). doi: 10.1128/mBio.00969-14. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/8nx4b02w, Digital.CSIC. Repositorio Institucional del CSIC, instname, Biblos-e Archivo. Repositorio Institucional de la UAM, mBio, Vol 5, Iss 2 (2014), mBio
- Publication Year :
- 2014
- Publisher :
- eScholarship, University of California, 2014.
-
Abstract
- For nearly 3 decades, listeriologists and immunologists have used mainly three strains of the same serovar (1/2a) to analyze the virulence of the bacterial pathogen Listeria monocytogenes. The genomes of two of these strains, EGD-e and 10403S, were released in 2001 and 2008, respectively. Here we report the genome sequence of the third reference strain, EGD, and extensive genomic and phenotypic comparisons of the three strains. Strikingly, EGD-e is genetically highly distinct from EGD (29,016 single nucleotide polymorphisms [SNPs]) and 10403S (30,296 SNPs), and is more related to serovar 1/2c than 1/2a strains. We also found that while EGD and 10403S strains are genetically very close (317 SNPs), EGD has a point mutation in the transcriptional regulator PrfA (PrfA*), leading to constitutive expression of several major virulence genes. We generated an EGD-e PrfA*mutant and showed that EGD behaves like this strain in vitro, with slower growth in broth and higher invasiveness in human cells than those of EGD-e and 10403S. In contrast, bacterial counts in blood, liver, and spleen during infection in mice revealed that EGD and 10403S are less virulent than EGD-e, which is itself less virulent than EGD-e PrfA*. Thus, constitutive expression of PrfA-regulated virulence genes does not appear to provide a significant advantage to the EGD strain during infection in vivo, highlighting the fact that in vitro invasion assays are not sufficient for evaluating the pathogenic potential of L. monocytogenes strains. Together, our results pave the way for deciphering unexplained differences or discrepancies in experiments using different L. monocytogenes strains. © 2014 Bécavin et al.<br />European Research Council (advanced grant 233348), the French Agence Nationale de la Recherche (grants BACNET 10-BINF-02-01, IBEID ANR-10-LABX-62-01, and ERA-NET ANR-2010-PATH), the Institut Pasteur, the Institut National de la Santé et de la Recherche Médicale, and the Institut National de la Recherche Agronomique. A.K
- Subjects :
- DNA, Bacterial
Bacterias patógenas
Molecular Sequence Data
Virulence
medicine.disease_cause
Microbiology
Genome
Mice
Listeria monocytogenes
Virology
medicine
Animals
Humans
Listeriosis
Pathogen
Gene
Genetics
Whole genome sequencing
Mice, Inbred BALB C
Mutation
biology
business.industry
Genetic Variation
Sequence Analysis, DNA
Biología y Biomedicina / Biología
biology.organism_classification
QR1-502
3. Good health
Disease Models, Animal
Blood
Liver
Listeria
business
Genome, Bacterial
Spleen
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Bécavin, C; Bouchier, C; Lechat, P; Archambaud, C; Creno, S; Gouin, E; et al.(2014). Comparison of widely used Listeria monocytogenes strains EGD, 10403S, and EGD-e highlights genomic differences underlying variations in pathogenicity. mBio, 5(2). doi: 10.1128/mBio.00969-14. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/8nx4b02w, Digital.CSIC. Repositorio Institucional del CSIC, instname, Biblos-e Archivo. Repositorio Institucional de la UAM, mBio, Vol 5, Iss 2 (2014), mBio
- Accession number :
- edsair.doi.dedup.....db0d35780569e925e3243a4292f9eae0