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An Outer Segment Localization Signal at the C Terminus of the Photoreceptor-Specific Retinol Dehydrogenase
- Source :
- The Journal of Neuroscience. 24:2623-2632
- Publication Year :
- 2004
- Publisher :
- Society for Neuroscience, 2004.
-
Abstract
- Photoreceptor retinol dehydrogenase (prRDH) is a membrane-associated cytosolic protein that localizes to the outer segments (OS) of rods and cones. Here, we demonstrate that the C-terminal 16 amino acids of prRDH confer membrane association as well as cone and rod OS targeting on a linked green fluorescent protein. Membrane association in transfected 293 cells and in transgenicXenopusphotoreceptors is mediated by fatty acylation at one or more evolutionarily conserved cysteines within the prRDH C-terminal tail. In bovine OS, native prRDH is similarly acylated, and hydrolysis of this linkage releases the protein from the membrane. Efficient OS localization requires both membrane association and the prRDH sequence... (V/I)XPX at the extreme C terminus, which closely resembles the C-terminal sequence that targets opsin/rhodopsin to the OS. Taken together, these data imply that the C-terminal... (V/I)XPX sequence is a general OS localization signal that can function in the context of both integral and peripheral membrane proteins. This strategy for OS localization resembles those used for protein localization to mitochondria, peroxisomes, endosomes, and endoplasmic reticulum; in each case, a short N- or C-terminal sequence is shared among structurally diverse proteins that are targeted to the same subcellular destination.
- Subjects :
- Vesicle-associated membrane protein 8
genetic structures
Recombinant Fusion Proteins
Amino Acid Motifs
Green Fluorescent Proteins
Molecular Sequence Data
Palmitic Acid
Biology
Transfection
medicine.disease_cause
Retinol dehydrogenase
Cell Line
Animals, Genetically Modified
Xenopus laevis
Protein targeting
medicine
Animals
Humans
Photoreceptor Cells
Amino Acid Sequence
Peptide sequence
Conserved Sequence
Zebrafish
General Neuroscience
Endoplasmic reticulum
Peripheral membrane protein
Rod Cell Outer Segment
Protein subcellular localization prediction
Alcohol Oxidoreductases
Luminescent Proteins
Protein Transport
Biochemistry
Larva
Cattle
sense organs
Fatty acylation
Cellular/Molecular
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....db1fb4dfa76d80b882ef037557f83488