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<scp>rIL</scp> ‐35 prevents murine transfusion‐related acute lung injury by inhibiting the activation of endothelial cells
- Source :
- Transfusion. 60:1434-1442
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Background Transfusion-related acute lung injury (TRALI) is an important cause of death associated with transfusion, and no specific clinical treatments are available. Endothelial cells are believed to play an important role in the development of TRALI. This study investigated whether IL-35, an endothelial stabilizing cytokine could regulate the severity of antibody-mediated TRALI in vivo. Study design and methods Human microvascular endothelial cells (HMVECs) were cultured in vitro, rIL-35(2 μg/mL) was added before HMVECs activation, and HMVECs were fully activated by LPS (0.5 μg/mL). Then cells were collected for flow cytometry analysis. We used a previously established "two-event" mouse model of TRALI with naive and lipopolysaccharide (LPS)-injected mice as controls. rIL-35(100 μg/kg) was injected into the tail vein for 3 consecutive days before the induction of the TRALI model. Samples were collected 2 hours after TRALI induction and tested for lung tissue myeloperoxidase activity, total protein levels, lung tissue histology, endothelial cell activation assay, and cytokine assay. Results In vitro culture of HMVECs with rIL-35 verified that rIL-35 inhibited endothelial cells. In a mouse model, prophylactic administration of rIL-35 prevented pulmonary edema, increased lung protein levels, and reduced polymorphonuclear neutrophil accumulation in the lung. Conclusions This work suggests that antibody-mediated murine TRALI can be prevented by rIL-35, and that rIL-35 appears to work by inhibiting the activation of lung endothelial cells.
- Subjects :
- Lipopolysaccharides
Male
Lipopolysaccharide
Neutrophils
medicine.medical_treatment
Immunology
030204 cardiovascular system & hematology
Lung injury
Pharmacology
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
In vivo
medicine
Animals
Humans
Immunology and Allergy
Lung
Mice, Inbred BALB C
business.industry
Interleukins
Endothelial Cells
Hematology
Pulmonary edema
medicine.disease
Recombinant Proteins
Endothelial stem cell
Disease Models, Animal
Transfusion-Related Acute Lung Injury
medicine.anatomical_structure
Cytokine
Neutrophil Infiltration
chemistry
business
030215 immunology
Transfusion-related acute lung injury
Subjects
Details
- ISSN :
- 15372995 and 00411132
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Transfusion
- Accession number :
- edsair.doi.dedup.....db4d7338b086b47af15d415931693d34