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Genome-wide long non-coding RNA association study on Han Chinese women identifies lncHSAT164 as a novel susceptibility gene for breast cancer
- Source :
- Chinese Medical Journal, Vol 134, Iss 10, Pp 1138-1145 (2021), Chinese Medical Journal
- Publication Year :
- 2021
- Publisher :
- Wolters Kluwer, 2021.
-
Abstract
- Background:. Single-nucleotide polymorphisms (SNPs)-associated genes and long non-coding RNAs (lncRNAs) can contribute to human disease. To comprehensively investigate the contribution of lncRNAs to breast cancer, we performed the first genome-wide lncRNA association study on Han Chinese women. Methods:. We designed an lncRNA array containing >800,000 SNPs, which was incorporated into a 96-array plate by Affymetrix (CapitalBio Technology, China). Subsequently, we performed a two-stage genome-wide lncRNA association study on Han Chinese women covering 11,942 individuals (5634 breast cancer patients and 6308 healthy controls). Additionally, in vitro gain or loss of function strategies were performed to clarify the function of a novel SNP-associated gene. Results:. We identified a novel breast cancer-associated susceptibility SNP, rs11066150 (Pmeta = 2.34 × 10−8), and a previously reported SNP, rs9397435 (Pmeta = 4.32 × 10−38), in Han Chinese women. rs11066150 is located in NONHSAT164009.1 (lncHSAT164), which is highly expressed in breast cancer tissues and cell lines. lncHSAT164 overexpression promoted colony formation, whereas lncHSAT164 knockdown promoted cell apoptosis and reduced colony formation by regulating the cell cycle. Conclusions:. Based on our lncRNA array, we identified a novel breast cancer-associated lncRNA and found that lncHSAT164 may contribute to breast cancer by regulating the cell cycle. These findings suggest a potential therapeutic target in breast cancer.
- Subjects :
- China
Single-nucleotide polymorphism
Genome-wide association study
Breast Neoplasms
Biology
Single-nucleotide polymorphisms
Polymorphism, Single Nucleotide
03 medical and health sciences
0302 clinical medicine
Breast cancer
Asian People
medicine
SNP
Humans
Genetic Predisposition to Disease
Gene
Gene knockdown
lncHSAT164
General Medicine
Original Articles
Cell cycle
medicine.disease
Long non-coding RNA
030220 oncology & carcinogenesis
Case-Control Studies
Cancer research
Medicine
Female
RNA, Long Noncoding
030217 neurology & neurosurgery
Genome-Wide Association Study
Subjects
Details
- Language :
- English
- ISSN :
- 25425641 and 03666999
- Volume :
- 134
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Chinese Medical Journal
- Accession number :
- edsair.doi.dedup.....db64df87e85c21c8e002c34ea57f6961