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Comprehensive analysis of lncRNA and mRNA based on expression microarray profiling reveals different characteristics of osteoarthritis between Tibetan and Han patients
- Source :
- Journal of Orthopaedic Surgery and Research, Vol 16, Iss 1, Pp 1-9 (2021), Journal of Orthopaedic Surgery and Research
- Publication Year :
- 2021
- Publisher :
- BMC, 2021.
-
Abstract
- Background Osteoarthritis (OA) is thought to be the most prevalent chronic joint disease, especially in Tibet of China. Here, we aimed to explore the integrative lncRNA and mRNA landscape between the OA patients of Tibet and Han. Methods The lncRNA and mRNA expression microarray profiling was performed by SurePrint G3 Human Gene Expression 8x60K v2 Microarray in articular cartilage samples from OA patients of Han nationality and Tibetans, followed by GO, KEGG, and trans-regulation and cis-regulation analysis of lncRNA and mRNA. Results We found a total of 117 lncRNAs and 297 mRNAs differently expressed in the cartilage tissues of Tibetans (n = 5) comparing with those of Chinese Han (n = 3), in which 49 lncRNAs and 158 mRNAs were upregulated, and 68 lncRNAs and 139 mRNAs were downregulated. GO and KEGG analysis showed that several unreported biological processes and signaling pathways were particularly identified. LncRNA-mRNA co-expression analysis revealed a remarkable lncRNA-mRNA relationship, in which OTOA may play a critical role in the different mechanisms of the OA progression between Tibetans and Chinese Han. Conclusion This study identified different lncRNA/mRNA expression profiling between OA patients of Tibetans and Han, which were involved in many characteristic biological processes and signaling pathways.
- Subjects :
- Cartilage, Articular
0301 basic medicine
China
lcsh:Diseases of the musculoskeletal system
Microarray
Gene Expression
Osteoarthritis
Computational biology
Tibet
03 medical and health sciences
0302 clinical medicine
Asian People
Downregulation and upregulation
lcsh:Orthopedic surgery
Gene expression
Humans
Medicine
Orthopedics and Sports Medicine
RNA, Messenger
KEGG
Messenger RNA
business.industry
Gene Expression Profiling
Microarray profiling
medicine.disease
lncRNA-mRNA
lcsh:RD701-811
Genetics, Population
030104 developmental biology
Tissue Array Analysis
030220 oncology & carcinogenesis
OTOA
RNA, Long Noncoding
Surgery
Signal transduction
lcsh:RC925-935
business
Research Article
Tibetan
Subjects
Details
- Language :
- English
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Orthopaedic Surgery and Research
- Accession number :
- edsair.doi.dedup.....db6b45a2b28afb8de41c783307c35c0e