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Anti-melanin deposition activity and active constituents of Jatropha multifida stems
- Source :
- Journal of natural medicines. 73(4)
- Publication Year :
- 2019
-
Abstract
- Jatropha multifida is a medicinal plant that belongs to the Euphorbiaceae family. Our investigation revealed that the chloroform extract of J. multifida stems showed anti-melanin deposition activity against α-melanocyte stimulating hormone (α-MSH)- and 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in the mouse melanoma cell line (B16-F10). Further fractionation and purification of the major constituents led to the isolation of two coumarins (1 and 2) and seven known lignoids (3–9). All isolated compounds exhibited anti-melanin deposition activities against the mouse melanoma cell line (B16-F10) with IC50 values ranging from 37.5 to 560.1 µM, without any cytotoxicity even at high concentrations, except for 8. Further mechanistic studies suggested that 9 downregulated tyrosinase mRNA expression, while the anti-melanin deposition activities of 4 and 8 appeared to be unrelated to tyrosinase inhibition and the downregulated expression of the key melanogenesis-associated mRNAs. These results suggested that J. multifida could possess potent skin whitening ingredients.
- Subjects :
- IBMX
Tyrosinase
Melanoma, Experimental
Down-Regulation
Jatropha
Fractionation
01 natural sciences
Melanin
chemistry.chemical_compound
Mice
Cell Line, Tumor
Animals
Cytotoxicity
Melanins
biology
010405 organic chemistry
Monophenol Monooxygenase
Plant Extracts
Euphorbiaceae
Skin whitening
biology.organism_classification
0104 chemical sciences
010404 medicinal & biomolecular chemistry
chemistry
Biochemistry
Cell culture
alpha-MSH
Molecular Medicine
Subjects
Details
- ISSN :
- 18610293
- Volume :
- 73
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of natural medicines
- Accession number :
- edsair.doi.dedup.....db7fc1db89d99c861cb50717039a6f01