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Expression of leptin receptor mRNA (long form splice variant) in the human cerebellum
- Source :
- Neuroreport, Vol. 8, No 14 (1997) pp. 3123-6
- Publication Year :
- 1997
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1997.
-
Abstract
- Primers specific to the long isoform of leptin receptor (OB-Rb) mRNA were used in reverse transcriptase-linked polymerase chain reactions (RT-PCR) to investigate the expression of this receptor in the hypothalamus and cerebellum of human and rat. For both regions, we observed RT-PCR cDNAs as well as restriction enzyme cleavage fragments of expected sizes. Additionally, in situ hybridization of human cerebellum using two independent [35S]oligonucleotide probes complementary to the OB-Rb mRNA sequence revealed a prominent hybridization signal within the granular layer. Overall, our findings demonstrate the expression of OB-Rb mRNA in the cerebellum and suggest that in such a location, leptin receptors may mediate a function presumably not linked to body weight homeostasis.
- Subjects :
- Gene isoform
Carrier Proteins/genetics
medicine.medical_specialty
RNA Splicing
Receptors, Cell Surface
In situ hybridization
Biology
ddc:616.89
ddc:590
Cerebellum
Internal medicine
Gene expression
medicine
Animals
Humans
Obesity
RNA, Messenger
Receptors, Cytokine
Receptor
In Situ Hybridization
Cerebellum/metabolism
Messenger RNA
Leptin receptor
Oligonucleotide
General Neuroscience
Receptors, Cytokine/genetics
Alternative splicing
RNA, Messenger/biosynthesis
Rats
Cell biology
Endocrinology
Receptors, Leptin
Carrier Proteins
Subjects
Details
- ISSN :
- 09594965
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- NeuroReport
- Accession number :
- edsair.doi.dedup.....dbc796851883c238811a06e8b570458c