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Crystal Structures of Cif from Bacterial Pathogens Photorhabdus luminescens and Burkholderia pseudomallei
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2009, 4 (5), pp.e5582. ⟨10.1371/journal.pone.0005582⟩, PLoS ONE, Vol 4, Iss 5, p e5582 (2009), Plos One 5 (4), e5582. (2009)
- Publication Year :
- 2009
- Publisher :
- Public Library of Science (PLoS), 2009.
-
Abstract
- International audience; A pre-requisite for bacterial pathogenesis is the successful interaction of a pathogen with a host. One mechanism used by a broad range of Gram negative bacterial pathogens is to deliver effector proteins directly into host cells through a dedicated type III secretion system where they modulate host cell function. The cycle inhibiting factor (Cif) family of effector proteins, identified in a growing number of pathogens that harbour functional type III secretion systems and have a wide host range, arrest the eukaryotic cell cycle. Here, the crystal structures of Cifs from the insect pathogen/nematode symbiont Photorhabdus luminescens (a γ-proteobacterium) and human pathogen Burkholderia pseudomallei (a β-proteobacterium) are presented. Both of these proteins adopt an overall fold similar to the papain sub-family of cysteine proteases, as originally identified in the structure of a truncated form of Cif from Enteropathogenic E. coli (EPEC), despite sharing only limited sequence identity. The structure of an N-terminal region, referred to here as the ‘tail-domain’ (absent in the EPEC Cif structure), suggests a surface likely to be involved in host-cell substrate recognition. The conformation of the Cys-His-Gln catalytic triad is retained, and the essential cysteine is exposed to solvent and addressable by small molecule reagents. These structures and biochemical work contribute to the rapidly expanding literature on Cifs, and direct further studies to better understand the molecular details of the activity of these proteins.
- Subjects :
- Burkholderia pseudomallei
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
Biophysics
lcsh:Medicine
Human pathogen
Crystallography, X-Ray
Biochemistry
Microbiology
Protein Structure, Secondary
Type three secretion system
03 medical and health sciences
Bacterial Proteins
Catalytic Domain
Photorhabdus luminescens
Secretion
Amino Acid Sequence
lcsh:Science
Pathogen
photorhabdus luminescens
030304 developmental biology
bactérie
0303 health sciences
Multidisciplinary
biology
030306 microbiology
Effector
lcsh:R
biology.organism_classification
Protein Structure, Tertiary
Chromatography, Gel
lcsh:Q
bactérie pathogène
Photorhabdus
agent pathogène
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....dbc9113d0d07d0b286b02f974a90a185
- Full Text :
- https://doi.org/10.1371/journal.pone.0005582