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Structure function relationships in three lipids A from the Ralstonia genus rising in obese patients
- Source :
- Biochimie 159, 72-80. (2019), Biochimie, Biochimie, Elsevier, 2019, 159 (159), pp.72-80. ⟨10.1016/j.biochi.2019.01.015⟩, Biochimie, Elsevier, 2019, 159, pp.72--80. ⟨10.1016/j.biochi.2019.01.015⟩, Biochimie, 2019, 159 (159), pp.72-80. ⟨10.1016/j.biochi.2019.01.015⟩
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- International audience; The identification of a functional molecular moiety relating the lipopolysaccharides (LPSs) to their capacity to induce inflammation-mediated metabolic diseases needed to be performed. We previously described a proportional increase in the relative abundance of the 16 SrDNA bacterial gene from the genus Ralstonia, within the microbiota from the adipose tissue stroma vascular fraction of obese patients, suggesting a causal role of the bacteria. Therefore, we first characterized the structures of the lipids A, the inflammatory inducing moieties of LPSs, of three Ralstonia species: Ralstonia eutropha, R. mannitolilytica and R. pickettii, and then compared each, in terms of in vitro inflammatory capacities. R. pickettii lipid A displaying only 5 Fatty Acids (FA) was a weaker inducer of inflammation, compared to the two other species harboring hexa-acylated lipids A, despite the presence of 2 AraN substituents on the phosphate groups. With regard to in vitro pro-inflammatory activities, TNF-alpha and IL-6 inducing capacities were compared on THP-1 cells treated with LPSs isolated from the three Ralstonia. R. pickettii, with low inflammatory capacities, and recently involved in nosocomial outcomes, could explain the low inflammatory level reported in previous studies on diabetic patients and animals. In addition, transmission electron microscopy was performed on the three Ralstonia species. It showed that the R. pickettii under-acylated LPSs, with a higher level of phosphate substitution had the capacity of producing more outer membrane vesicles (OMVs). The latter could facilitate transfer of LPSs to the blood and explain the increased low-grade inflammation observed in obese/diabetic patients. (C) 2019 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
- Subjects :
- 0301 basic medicine
Lipopolysaccharide
onset
THP-1 Cells
[SDV]Life Sciences [q-bio]
Ralstonia
Structure
Mass spectrometry
Cytokines
Metabolic diseases
Adipose tissue
Inflammation
Biochemistry
Microbiology
strains
Lipid A
Structure-Activity Relationship
03 medical and health sciences
chemistry.chemical_compound
microextraction
medicine
Humans
Inducer
Obesity
phosphate groups
bacteria
lps
030102 biochemistry & molecular biology
biology
endotoxemia
General Medicine
lipopolysaccharides
biology.organism_classification
3. Good health
[SDV] Life Sciences [q-bio]
030104 developmental biology
chemistry
lipids (amino acids, peptides, and proteins)
medicine.symptom
Bacterial outer membrane
Bacteria
Autre (Sciences du Vivant)
Subjects
Details
- ISSN :
- 03009084
- Volume :
- 159
- Database :
- OpenAIRE
- Journal :
- Biochimie
- Accession number :
- edsair.doi.dedup.....dbd33b327c1a21853392bfbc09189244