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A phase I trial of DNA vaccination with a plasmid expressing prostate-specific antigen in patients with hormone-refractory prostate cancer
A phase I trial of DNA vaccination with a plasmid expressing prostate-specific antigen in patients with hormone-refractory prostate cancer
- Source :
- British Journal of Cancer
- Publication Year :
- 2004
-
Abstract
- Prostate-specific antigen (PSA) is a serine protease secreted at low levels by normal luminal epithelial cells of the prostate and in significantly higher levels by prostate cancer cells. Therefore, PSA is a potential target for various immunotherapeutical approaches against prostate cancer. DNA vaccination has been investigated as immunotherapy for infectious diseases in patients and for specific treatment of cancer in certain animal models. In animal studies, we have demonstrated that vaccination with plasmid vector pVAX/PSA results in PSA-specific cellular response and protection against tumour challenge. The purpose of the trial was to evaluate the safety, feasibility and biological efficacy of pVAX/PSA vaccine in the clinic. A phase I trial of pVAX/PSA, together with cytokine granulocyte/macrophage-colony stimulating factor (GM-CSF) (Molgramostim) and IL-2 (Aldesleukin) as vaccine adjuvants, was carried out in patients with hormone-refractory prostate cancer. To evaluate the biologically active dose, the vaccine was administered during five cycles in doses of 100, 300 and 900 microg, with three patients in each cohort. Eight patients were evaluable. A PSA-specific cellular immune response, measured by IFN-gamma production against recombinant PSA protein, and a rise in anti-PSA IgG were detected in two of three patients after vaccination in the highest dose cohort. A decrease in the slope of PSA was observed in the two patients exhibiting IFN-gamma production to PSA. No adverse effects (WHO grade >2) were observed in any dose cohort. We demonstrate that DNA vaccination with a PSA-coding plasmid vector, given with GM-CSF and IL-2 to patients with prostate cancer, is safe and in doses of 900 microg the vaccine can induce cellular and humoral immune responses against PSA protein.
- Subjects :
- PCA3
Oncology
Male
DNA vaccine
Cancer Research
medicine.medical_specialty
medicine.medical_treatment
Genetic Vectors
Adenocarcinoma
urologic and male genital diseases
Cancer Vaccines
immune response
IFN-gamma
Prostate cancer
Clinical
Prostate
Aldesleukin
Internal medicine
Vaccines, DNA
Medicine
Humans
prostate-specific antigen
Aged
Aged, 80 and over
Immunity, Cellular
Dose-Response Relationship, Drug
business.industry
Cancer
Granulocyte-Macrophage Colony-Stimulating Factor
Prostatic Neoplasms
Immunotherapy
Middle Aged
medicine.disease
prostate cancer
Vaccination
Prostate-specific antigen
medicine.anatomical_structure
Treatment Outcome
Drug Resistance, Neoplasm
Immunology
Antibody Formation
Interleukin-2
immunotherapy
business
Plasmids
Subjects
Details
- ISSN :
- 00070920
- Volume :
- 91
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- British journal of cancer
- Accession number :
- edsair.doi.dedup.....dbdfdc484a921649629c51fb5b996735