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Presence of gonadotropin-releasing hormone (GnRH) and its mRNA in rat pancreas
- Source :
- Molecular and cellular endocrinology. 172(1-2)
- Publication Year :
- 2001
-
Abstract
- Gonadotropin-releasing hormone (GnRH) has been reported to exist in many non-hypothalamic tissues, such as the placenta, gonads, and mammary glands, while there still have been no reports concerning the existence and expression of GnRH in the mammalian digestive system. Immunocytochemistry and in situ hybridization results show that GnRH molecule and GnRH mRNA are both exclusively distributed in exocrine pancreas, and RT-PCR result further proves that GnRH transcription units do exist in the pancreas, which possess the same sequence as the hypothalamus GnRH mRNA. Quantitative analysis indicates that mRNA levels in rat pancreas remain at a low level (less than 10% of that in hypothalamus) without sexual or developmental difference. This is the first report suggesting the existence and gene expression of GnRH in rat pancreas.
- Subjects :
- Male
endocrine system
medicine.medical_specialty
Immunocytochemistry
In situ hybridization
Gonadotropin-releasing hormone
Biology
Biochemistry
Gonadotropin-Releasing Hormone
Rats, Sprague-Dawley
Endocrinology
Sex Factors
Internal medicine
Gene expression
medicine
Animals
Protein Isoforms
Tissue Distribution
RNA, Messenger
Molecular Biology
Pancreas
In Situ Hybridization
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Immunohistochemistry
Rats
medicine.anatomical_structure
Hypothalamus
Female
hormones, hormone substitutes, and hormone antagonists
Hormone
Subjects
Details
- ISSN :
- 03037207
- Volume :
- 172
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular endocrinology
- Accession number :
- edsair.doi.dedup.....dbf774820e5fef66cfc3eba724a40d28