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Characterization of CaHsp70-1, a pepper heat-shock protein gene in response to heat stress and some regulation exogenous substances in Capsicum annuum L
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 15, Iss 11, Pp 19741-19759 (2014), Volume 15, Issue 11, Pages 19741-19759
- Publication Year :
- 2014
-
Abstract
- Pepper (Capsicum annuum L.) is sensitive to heat stress (HS). Heat shock proteins 70 (Hsp70s) play a crucial role in protecting plant cells against HS and control varies characters in different plants. However, CaHsp70-1 gene was not well characterized in pepper. In this study, CaHsp70-1 was cloned from the pepper thermotolerant line R9, which encoded a protein of 652 amino acids, with a molecular weight of 71.54 kDa and an isoelectric point of 5.20. CaHsp70-1 belongs to the cytosolic Hsp70 subgroup, and best matched with tomato SlHsp70. CaHsp70-1 was highly induced in root, stem, leaf and flower in R9 with HS treatment (40 °C for 2 h). In both thermosensitive line B6 and thermotolerant line R9, CaHsp70-1 significantly increased after 0.5 h of HS (40 °C), and maintained in a higher level after 4 h HS. The expression of CaHsp70-1 induced by CaCl2, H2O2 and putrescine (Put) under HS were difference between B6 and R9 lines. The different expression patterns may be related to the differences in promoters of CaHsp70-1 from the two lines. These results suggest that CaHsp70-1 as a member of cytosolic Hsp70 subgroup, may be involved in HS defense response via a signal transduction pathway contained Ca2+, H2O2 and Put.
- Subjects :
- Cytoplasm
Capsicum annuum L
CaHsp70-1
lcsh:Chemistry
heat stress
chemistry.chemical_compound
Calcium Chloride
Gene Expression Regulation, Plant
Gene expression
Promoter Regions, Genetic
lcsh:QH301-705.5
Spectroscopy
Plant Proteins
chemistry.chemical_classification
Regulation of gene expression
Temperature
food and beverages
General Medicine
Computer Science Applications
Amino acid
Capsicum
Molecular Sequence Data
Biology
Catalysis
Article
Cell Line
Inorganic Chemistry
Heat shock protein
Plant Cells
Pepper
Botany
Putrescine
HSP70 Heat-Shock Proteins
Physical and Theoretical Chemistry
Molecular Biology
Cell Nucleus
Base Sequence
Organic Chemistry
Hydrogen Peroxide
Molecular biology
Hsp70
Molecular Weight
Plant Leaves
Isoelectric point
chemistry
lcsh:Biology (General)
lcsh:QD1-999
gene expression
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 15
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- International journal of molecular sciences
- Accession number :
- edsair.doi.dedup.....dc102ca8884b9314a83de1fe930a2d83