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LIME
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 2003
- Publisher :
- The Rockefeller University Press, 2003.
-
Abstract
- Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. Thus, LIME appears to be involved in regulation of T cell activation by coreceptors.
- Subjects :
- DNA, Complementary
CD8 Antigens
Immunology
Molecular Sequence Data
chemical and pharmacologic phenomena
Biology
Jurkat cells
complex mixtures
SH3 domain
Article
CSK Tyrosine-Protein Kinase
03 medical and health sciences
0302 clinical medicine
Membrane Microdomains
Csk
Immunology and Allergy
Humans
Src family kinase
Amino Acid Sequence
Databases, Protein
Lipid raft
030304 developmental biology
0303 health sciences
Tyrosine-protein kinase CSK
phosphorylation
Membrane raft
Signal transducing adaptor protein
hemic and immune systems
Protein-Tyrosine Kinases
Cell biology
Lck
Adaptor Proteins, Vesicular Transport
src-Family Kinases
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
CD4 Antigens
Signal transduction
signal transduction
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 15409538 and 00221007
- Volume :
- 198
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- The Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....dc17ae6253fcedff484e1c2891a09e2c