Dietary black cumin (Nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of Nile tilapia (Oreochromis niloticus) infected by Burkholderia cepacia

The present study assessed the protective aspects of the dietary exposure to black cumin (Nigella sativa, NS) powder on immune response, liver and kidney enzymes, tissue antioxidant capacity, gene expression, and testing the antibacterial activity against Burkholderia cepacia (B. cepacia, BC). Nile tilapia (O. niloticus) (n = 150) were divided into 5 groups, the first was control (Cont) neither challenged nor treated, and fed a basal diet for 59 days. The second one received 30 g kg−1 of NS-incorporated diet and farmed for 45 days (45NS). The third (Cont-BC) and the fourth (14NS-BC) groups were fed a basal diet, while the fifth (59NS-BC) was fed NS-supplemented diet for 45 days. Then, the last three groups were intraperitoneally inoculated with 0.2 mL of virulent BC, and kept under observation for 14 days. During the observation period, Cont-BC group was continuously received a basal diet, while 14NS-BC and 59NS-BC were fed on NS-supplemented diet. Results showed that fish received NS-dietary supplement exhibited significantly (P