Back to Search
Start Over
Single particle electron microscopy
- Source :
- Photosynthesis Research
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structures at low, medium, and high resolution. Since electron micrographs of biological objects are very noisy, improvement of the signal-to-noise ratio by image processing is an integral part of EM, and this is performed by averaging large numbers of individual projections. Averaging procedures can be divided into crystallographic and non-crystallographic methods. The crystallographic averaging method, based on two-dimensional (2D) crystals of (membrane) proteins, yielded in solving atomic protein structures in the last century. More recently, single particle analysis could be extended to solve atomic structures as well. It is a suitable method for large proteins, viruses, and proteins that are difficult to crystallize. Because it is also a fast method to reveal the low-to-medium resolution structures, the impact of its application is growing rapidly. Technical aspects, results, and possibilities are presented.
- Subjects :
- STRUCTURAL-CHARACTERIZATION
Models, Molecular
THERMOSYNECHOCOCCUS-ELONGATUS
CRYOMICROSCOPY
Analytical chemistry
ANGSTROM RESOLUTION
Single particle analysis
Image processing
Review
Plant Science
Negative Staining
Biochemistry
law.invention
Protein structure
law
ATOMIC-RESOLUTION
Electron microscopy
Photosynthesis
LIGHT-HARVESTING COMPLEX
MEMBRANE-PROTEINS
Chemistry
Cryoelectron Microscopy
Resolution (electron density)
Cell Biology
General Medicine
Negative stain
Computational physics
MODEL
Microscopy, Electron
Membrane protein
PHOTOSYSTEM-I
Multiprotein Complexes
Particle
SUPRAMOLECULAR ORGANIZATION
Electron microscope
Subjects
Details
- ISSN :
- 15735079 and 01668595
- Volume :
- 102
- Database :
- OpenAIRE
- Journal :
- Photosynthesis Research
- Accession number :
- edsair.doi.dedup.....dc41290909e2572c9bbef8f0625bb116