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Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut
Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut
- Source :
- Protein Science, 29(7), 1581-1595, Protein Science : A Publication of the Protein Society, Protein Science 29 (2020) 7, Protein Science, 29(7), 1581-1595. Wiley-Blackwell
- Publication Year :
- 2020
-
Abstract
- Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis‐related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA‐seq analysis, we were able to identify several PR10‐like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography–mass spectrometry (LC–MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.
- Subjects :
- Bioinformatics analysis
Full‐length Papers
In silico
Biochemie
RNA-Seq
Biology
Biochemistry
Protein expression
Article
oral allergy syndrome (OAS)
03 medical and health sciences
Tandem Mass Spectrometry
Levensmiddelenchemie
Nuts
Anacardium
Computer Simulation
Laboratorium voor Plantenfysiologie
Cashew nut
Molecular Biology
Gene
PR10
030304 developmental biology
Plant Proteins
VLAG
Food, Health & Consumer Research
0303 health sciences
Food Chemistry
030302 biochemistry & molecular biology
digestive, oral, and skin physiology
food and beverages
Allergens
cashew nut
Health & Consumer Research
Food
Anacardium occidentale
in silico allergenicity analysis
BIOS Applied Metabolic Systems
RNA‐seq
Bet v 1‐like
EPS
RNA-seq
Laboratory of Plant Physiology
Bet v 1-like
Chromatography, Liquid
Subjects
Details
- Language :
- English
- ISSN :
- 09618368
- Volume :
- 29
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Protein Science
- Accession number :
- edsair.doi.dedup.....dc49c8c53944a2c5322682499d1712d0
- Full Text :
- https://doi.org/10.1002/pro.3856