Back to Search Start Over

Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut

Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut

Authors :
Maria Consuelo Pina-Pérez
Harry J. Wichers
Shanna Bastiaan-Net
Jurriaan J. Mes
Renata M.C. Ariëns
Adrie H. Westphal
Antoine H. P. America
Nicolette W. de Jong
Bas J.W. Dekkers
Internal Medicine
Source :
Protein Science, 29(7), 1581-1595, Protein Science : A Publication of the Protein Society, Protein Science 29 (2020) 7, Protein Science, 29(7), 1581-1595. Wiley-Blackwell
Publication Year :
2020

Abstract

Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis‐related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA‐seq analysis, we were able to identify several PR10‐like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography–mass spectrometry (LC–MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.

Details

Language :
English
ISSN :
09618368
Volume :
29
Issue :
7
Database :
OpenAIRE
Journal :
Protein Science
Accession number :
edsair.doi.dedup.....dc49c8c53944a2c5322682499d1712d0
Full Text :
https://doi.org/10.1002/pro.3856