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FABP5 regulates the proliferation of clear cell renal cell carcinoma cells via the PI3K/AKT signaling pathway
- Source :
- International Journal of Oncology
- Publication Year :
- 2019
- Publisher :
- Spandidos Publications, 2019.
-
Abstract
- Clear cell renal cell carcinoma (ccRCC) has been associated with one of the highest mortality rates among all cancers. Fatty acid binding proteins (FABPs) are 14‑15 kDa proteins that are highly abundant in the cytosol of most tissues. FABP5, a member of the FABP family, has been observed to promote tumor cell growth in numerous cancer types. In order to investigate the function of FABP5 in ccRCC cells in the present study, RNA sequencing data from The Cancer Genome Atlas were analyzed to determine the expression levels of FABP5 in ccRCC patient samples. Survival and Cox regression analyses were performed to measure the association between FABP5 expression and clinicopathological features of patients with ccRCC. Subsequent in vitro experiments downregulated or overexpressed FABP5 in Caki‑1 and 786O ccRCC cells using lentiviral vectors to evaluate cell proliferation ability, and a xenograft transplantation model was established to examine the effect of FABP5 on tumorigenesis in vivo. The results demonstrated that FABP5 expression was significantly upregulated in samples from patients with ccRCC when compared with normal tissue samples. High FABP5 expression was also significantly correlated with tumor and metastasis classifications and predicted poor survival in patients with ccRCC. In ccRCC cells, silencing of FABP5 significantly inhibited cell proliferation, while overexpression of FABP5 promoted cell proliferation when compared to the respective controls. In addition, treatment with the phosphatidylinositol‑4,5‑bisphosphate 3‑kinase (PI3K)/AKT inhibitor, LY294002, attenuated the pro‑proliferative effects of exogenous FABP5 expression in Caki‑1 and 786O cells. This indicated that the PI3K/AKT signaling pathway may be partially involved in the FABP5‑mediated increase in ccRCC cell proliferation. Furthermore, FABP5 was observed to regulate tumor growth in nude mice in vivo. In conclusion, the results of the present study suggest that FABP5 may exert a pro‑proliferative role in ccRCC and may be associated with malignant progression and tumorigenesis.
- Subjects :
- Male
0301 basic medicine
Cancer Research
Morpholines
proliferation
Cell
Mice, Nude
clear cell renal cell carcinoma
Biology
Fatty Acid-Binding Proteins
medicine.disease_cause
Phosphatidylinositol 3-Kinases
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
phosphatidylinositol-4,5-bisphosphate 3-kinase/AKT signaling pathway
medicine
Animals
Humans
Carcinoma, Renal Cell
PI3K/AKT/mTOR pathway
Cell Proliferation
Phosphoinositide-3 Kinase Inhibitors
Oncogene
Cell growth
Akt/PKB signaling pathway
fatty acid binding protein 5
Computational Biology
Articles
Middle Aged
Cell cycle
Prognosis
medicine.disease
Survival Analysis
Xenograft Model Antitumor Assays
Kidney Neoplasms
Up-Regulation
Clear cell renal cell carcinoma
030104 developmental biology
medicine.anatomical_structure
Oncology
Chromones
030220 oncology & carcinogenesis
Cancer research
Female
Carcinogenesis
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 17912423 and 10196439
- Database :
- OpenAIRE
- Journal :
- International Journal of Oncology
- Accession number :
- edsair.doi.dedup.....dc55df9505d78ccd55a032ce15f508a3