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Leaf apoplastic alkalization promotes transcription of the ABA-synthesizing enzyme Vp14 and stomatal closure in Zea mays
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2020
-
Abstract
- The chloride component of NaCl salinity causes the leaf apoplast to transiently alkalinize. This transition in pH reduces stomatal aperture. However, whether this apoplastic pH (pHapo) transient initiates stomatal closure by interacting with other chloride stress-induced responses or whether the pH transient alone initiates stomatal closure is unknown. To clarify the problem, the transient alkalinization of the leaf apoplast was mimicked in intact maize (Zea mays L.) by infiltrating near-neutral pH buffers into the leaf apoplast. Effects of the pHapo transient could thus be investigated independently from other chloride stress-derived effects. Microscopy-based ratiometric live pHapo imaging was used to monitor pHapoin planta. LC-MS/MS and real-time quantitative reverse transcription–PCR leaf analyses showed that the artificially induced pHapo transient led to an increase in the concentrations of the stomata-regulating plant hormone abscisic acid (ABA) and in transcripts of the key ABA-synthesizing gene ZmVp14 in the leaf. Since stomatal aperture and stomatal conductance decreased according to pHapo, we conclude that the pHapo transient alone initiates stomatal closure. Therefore, the functionality does not depend on interactions with other compounds induced by chloride stress. Overall, our data indicate that the pH of the leaf apoplast links chloride salinity with the control of stomatal aperture via effects exerted on the transcription of ABA.<br />In maize, the transient alkalinization of the leaf apoplast acts as a switch that increases transcription of the ABA-synthesizing key gene viviparous 14 and reduces transpiration.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Stomatal conductance
chloride
Physiology
stomata
Plant Science
01 natural sciences
Chloride
Zea mays
salinity
transpiration
03 medical and health sciences
chemistry.chemical_compound
Tandem Mass Spectrometry
Guard cell
medicine
Abscisic acid
Transpiration
biology
AcademicSubjects/SCI01210
fungi
food and beverages
NCED
biology.organism_classification
apoplast
Research Papers
Apoplast
Salinity
Plant Leaves
030104 developmental biology
chemistry
ABA
Plant—Environment Interactions
Plant Stomata
Biophysics
Plant hormone
guard cell
alkalinization
010606 plant biology & botany
medicine.drug
Abscisic Acid
Chromatography, Liquid
Subjects
Details
- ISSN :
- 14602431
- Volume :
- 72
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of experimental botany
- Accession number :
- edsair.doi.dedup.....dc99df5fc2339b8e23121751514249fa