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The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin

Authors :
Sara Quintana-Sanchez
Natalia Gómez-Casanova
Javier Sánchez-Nieves
Rafael Gómez
Jarosław Rachuna
Sławomir Wąsik
Jacek Semaniak
Barbara Maciejewska
Zuzanna Drulis-Kawa
Karol Ciepluch
F. Javier de la Mata
Michał Arabski
Source :
International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1873, International Journal of Molecular Sciences, Vol 23, Iss 1873, p 1873 (2022)
Publication Year :
2022
Publisher :
Multidisciplinary Digital Publishing Institute, 2022.

Abstract

The search for new microbicide compounds is of an urgent need, especially against difficult-to-eradicate biofilm-forming bacteria. One attractive option is the application of cationic multivalent dendrimers as antibacterials and also as carriers of active molecules. These compounds require an adequate hydrophilic/hydrophobic structural balance to maximize the effect. Herein, we evaluated the antimicrobial activity of cationic carbosilane (CBS) dendrimers unmodified or modified with polyethylene glycol (PEG) units, against planktonic and biofilm-forming P. aeruginosa culture. Our study revealed that the presence of PEG destabilized the hydrophilic/hydrophobic balance but reduced the antibacterial activity measured by microbiological cultivation methods, laser interferometry and fluorescence microscopy. On the other hand, the activity can be improved by the combination of the CBS dendrimers with endolysin, a bacteriophage-encoded peptidoglycan hydrolase. This enzyme applied in the absence of the cationic CBS dendrimers is ineffective against Gram-negative bacteria because of the protective outer membrane shield. However, the endolysin—CBS dendrimer mixture enables the penetration through the membrane and then deterioration of the peptidoglycan layer, providing a synergic antimicrobial effect.

Details

Language :
English
ISSN :
14220067
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1873
Accession number :
edsair.doi.dedup.....dcb364050f14586ae9bd03e8a508deb7
Full Text :
https://doi.org/10.3390/ijms23031873