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Detection of herpesvirus and adenovirus co-infections with diagnostic DNA-microarrays
- Source :
- Journal of Virological Methods. 155:161-166
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- In immunocompromised patients, the diagnosis of infections with herpesviruses and adenoviruses relies mainly on PCR amplification of viral genomic DNA from clinical samples. In the case of co-infections with two or more viruses, single amplification of viral DNA from clinical samples has proven to be time-consuming and expensive, hampering the efficient diagnosis and therapy of viral co-infections. In this study, a diagnostic DNA-microarray allowing simultaneous detection of herpes simplex virus types 1 and 2 (HSV 1/2), varicella zoster virus (VZV), Epstein–Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus-6 types A and B (HHV-6 A/B), and adenovirus in clinical samples was developed and validated. The assay displays a high analytical sensitivity (10 genome equivalents (GE)/reaction) and specificity, being cost-effective and time-saving. Because the DNA-microarray uses the same analytical conditions as real-time quantitative PCR, it can be used as a screening device for multiple viral infections, followed by selective viral load measurement depending on the clinical context. Those features make the DNA-microarray an attractive device for the management of viral infections in immunocompromised patients.
- Subjects :
- viruses
Biology
medicine.disease_cause
Sensitivity and Specificity
Virus
law.invention
Adenovirus Infections, Human
Immunocompromised Host
law
Virology
medicine
Humans
Herpesviridae
Polymerase chain reaction
Oligonucleotide Array Sequence Analysis
Viral culture
Adenoviruses, Human
Varicella zoster virus
Cytomegalovirus
Herpesviridae Infections
Viral Load
Herpes simplex virus
Real-time polymerase chain reaction
DNA, Viral
Immunology
Viral load
Stem Cell Transplantation
Subjects
Details
- ISSN :
- 01660934
- Volume :
- 155
- Database :
- OpenAIRE
- Journal :
- Journal of Virological Methods
- Accession number :
- edsair.doi.dedup.....dcfcaebcdfc6985f791254c9cb08bb29