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Simultaneous quantitation of 23 bioactive compounds in Tanreqing capsule by highâperformance liquid chromatography electrospray ionization tandem mass spectrometry
- Source :
- Biomedical Chromatography. 33:e4531
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Tanreqing capsule (TRQC) is a formulation frequently used in traditional Chinese medicine to treat pyrexia, cough, expectoration and pharyngalgia. Since the pharmacological action of traditional Chinese medicines is closely related to their complex and diverse constituents, understanding the exact composition of TRQC is important to elucidate its clinical effectiveness and mechanism of action as well as to establish quality control methods and resolve safety issues. Herein, we employed high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry for the simultaneous quantitation of 23 bioactive compounds in five batches of TRQC; the analytes could be categorized into five types: organic acids (seven compounds), flavonoids (10 compounds), iridoids (two compounds), phenylethanoid glycosides (two compounds) and bile acids (two compounds). The calibration curves for all analytes showed good linearity (r > 0.9953), and the inter- and intra-day precisions did not exceed 4.94 and 4.97%, respectively. The recoveries varied from 90.47% to 109.80%; the corresponding relative standard deviations (RSDs) did not exceed 4.94%; and the repeatability (RSD < 4.72%) and stability (RSD < 4.88%) were also within acceptable limits. Thus, this study can be viewed as a fundamental reference for setting comprehensive TRQC quality standards.
- Subjects :
- Analyte
Calibration curve
Electrospray ionization
Clinical Biochemistry
Acids, Carbocyclic
Tandem mass spectrometry
030226 pharmacology & pharmacy
01 natural sciences
Biochemistry
High-performance liquid chromatography
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Limit of Detection
Tandem Mass Spectrometry
Drug Discovery
Iridoids
Glycosides
Molecular Biology
Chromatography, High Pressure Liquid
Flavonoids
Pharmacology
Chromatography
Chemistry
010401 analytical chemistry
Reproducibility of Results
General Medicine
Repeatability
Phenylethanoid
0104 chemical sciences
Linear Models
Quantitative analysis (chemistry)
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 10990801 and 02693879
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Biomedical Chromatography
- Accession number :
- edsair.doi.dedup.....dcfe575083860018bd138b45b4a171c3