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Biochemical markers of the mechanical quality of engineered hyaline cartilage
- Source :
- Journal of Materials Science: Materials in Medicine. 18:273-281
- Publication Year :
- 2007
- Publisher :
- Springer Science and Business Media LLC, 2007.
-
Abstract
- PUBLISHED<br />The aim of this study was to determine whether or not biochemical markers can be used as surrogate measures for the mechanical quality of tissue engineered cartilage. The biochemical composition of tissue engineered cartilage constructs were altered by varying either (i) the initial cell seeding density of the scaffold (seeding density protocol) or (ii) the length of time the engineered tissue was cultured (culture period protocol). The aggregate or Young?s moduli of the constructs were measured (by confined or unconfined compression respectively), and compared with the composition of the extracellular matrix by quantitative measurement of the glycosaminoglycan (GAG), hydroxyproline, collagen I and collagen II and collagen cross-links. The aggregate modulus correlated positively with both GAG and collagen II content, but not with collagen I content. Young?s modulus correlated positively with GAG, collagen II and collagen I content, and the ratio of mature to immature cross-links, but not with hydroxyproline content. These results suggested that hydroxyproline may be an unreliable indicator of mechanical quality of tissue engineered cartilage, and that a measure of collagen II and GAG content is required to predict the biomechanical quality of tissue engineered cartilage.<br />This work was funded by the European Union (EU) under the SCAFCART project (contract G5RD-CT-1999-00050). APH is funded in part by an endowed chair from the UK Arthritis Research Campaign.
- Subjects :
- Scaffold
Materials science
Statistics as Topic
Biomedical Engineering
Biophysics
Aggregate modulus
Bioengineering
Biomaterials
Glycosaminoglycan
Extracellular matrix
Hydroxyproline
chemistry.chemical_compound
Chondrocytes
Tissue engineering
Materials Testing
medicine
Animals
GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries)
Cells, Cultured
Biochemical markers
Glycosaminoglycans
Tissue Engineering
Hyaline cartilage
Cell Differentiation
Administrative Staff Authors
Elasticity
Hyaline Cartilage
medicine.anatomical_structure
chemistry
Cattle
Collagen
Stress, Mechanical
Biomarkers
Biomedical engineering
Subjects
Details
- ISSN :
- 15734838 and 09574530
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Journal of Materials Science: Materials in Medicine
- Accession number :
- edsair.doi.dedup.....dd133164520eb25740470192251043b6