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An sRNA Screen for Reversal of Quinolone Resistance in Escherichia coli
- Source :
- G3: Genes, Genomes, Genetics, Vol 10, Iss 1, Pp 79-88 (2020), G3: Genes|Genomes|Genetics
- Publication Year :
- 2020
- Publisher :
- Oxford University Press, 2020.
-
Abstract
- In light of the rising prevalence of antimicrobial resistance (AMR) and the slow pace of new antimicrobial development, there has been increasing interest in the development of adjuvants that improve or restore the effectiveness of existing drugs. Here, we use a novel small RNA (sRNA) screening approach to identify genes whose knockdown increases ciprofloxacin (CIP) sensitivity in a resistant strain of Escherichia coli. 5000 sRNA constructs were initially screened on a gyrA S83L background, ultimately leading to 30 validated genes whose disruption reduces CIP resistance. This set includes genes involved in DNA replication, repair, recombination, efflux, and other regulatory systems. Our findings increase understanding of the functional interactions of DNA Gyrase, and may aid in the development of new therapeutic approaches for combating AMR.
- Subjects :
- bacterial small rna
Biology
QH426-470
medicine.disease_cause
fluoroquinolone
DNA gyrase
03 medical and health sciences
Bacterial small RNA
Antibiotic resistance
adjuvant
Ciprofloxacin
Drug Resistance, Bacterial
drug targets
medicine
Genetics
antimicrobial resistance
Molecular Biology
Escherichia coli
Gene
Genetics (clinical)
030304 developmental biology
0303 health sciences
Gene knockdown
030306 microbiology
DNA replication
Mutant Screen Report
Reverse Genetics
Anti-Bacterial Agents
DNA Gyrase
Genes, Bacterial
RNA Interference
Efflux
escherichia coli
Subjects
Details
- Language :
- English
- ISSN :
- 21601836
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- G3: Genes, Genomes, Genetics
- Accession number :
- edsair.doi.dedup.....dd18b795362c3094d22ababefea1bcde