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Gene Expression Analysis to Investigate Biological Networks Underlying Nasal Inflammatory Dysfunctions Induced by Diesel Exhaust Particles Using an In Vivo System
- Source :
- Annals of Otology, Rhinology & Laryngology. 129:245-255
- Publication Year :
- 2019
- Publisher :
- SAGE Publications, 2019.
-
Abstract
- Objectives: Diesel exhaust particles (DEP)s are notorious ambient pollutants composed of a complex mixture of a carbon core and diverse chemical irritants. Several studies have demonstrated significant relationships between DEP exposure and serious nasal inflammatory response in vitro, but available information regarding underlying networks in terms of gene expression changes has not sufficiently explained potential mechanisms of DEP-induced nasal damage, especially in vivo. Methods: In the present study, we identified DEP-induced gene expression profiles under short-term and long-term exposure, and identified signaling pathways based on microarray data for understanding effects of DEP exposure in the mouse nasal cavity. Results: Alteration in gene expression due to DEP exposure provokes an imbalance of the immune system via dysregulated inflammatory markers, predicted to disrupt protective responses against harmful exogenous substances in the body. Several candidate markers were identified after validation using qRT-PCR, including S100A9, CAMP, IL20, and S100A8. Conclusions: Although further mechanistic studies are required for verifying the utility of the potential biomarkers suggested by the present study, our in vivo results may provide meaningful suggestions for understanding the complex cellular signaling pathways involved in DEP-induced nasal damages.
- Subjects :
- 0301 basic medicine
Nasal Provocation Tests
Diesel exhaust
Gene Expression
Real-Time Polymerase Chain Reaction
Tumor Necrosis Factor Receptor Superfamily, Member 9
03 medical and health sciences
0302 clinical medicine
Cathelicidins
In vivo
Gene expression
Animals
Calgranulin B
Medicine
Calgranulin A
Oligonucleotide Array Sequence Analysis
Rhinitis
Vehicle Emissions
Pollutant
Mice, Inbred BALB C
business.industry
Interleukins
General Medicine
respiratory system
030104 developmental biology
Otorhinolaryngology
Models, Animal
B7-1 Antigen
Biophysics
RNA
business
Biomarkers
030217 neurology & neurosurgery
Biological network
Chemical irritants
Antimicrobial Cationic Peptides
Signal Transduction
Subjects
Details
- ISSN :
- 1943572X and 00034894
- Volume :
- 129
- Database :
- OpenAIRE
- Journal :
- Annals of Otology, Rhinology & Laryngology
- Accession number :
- edsair.doi.dedup.....dd6ab0c422089f309604498245542df4