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Combined treatment with C16 peptide and angiopoietin-1 confers neuroprotection and reduces inflammation in 3-nitropropionic acid-induced dystonia mice
- Source :
- Aging (Albany NY)
- Publication Year :
- 2021
- Publisher :
- Impact Journals, 2021.
-
Abstract
- Dystonia is a disorder associated with abnormalities in many brain regions including the basal ganglia and cerebellum. The toxin 3-Nitropropionic acid (3-NP) can induce neuropathologies in the mice striatum and nigra substance, including excitotoxicity, neuroinflammation, and extensive neuronal atrophy, characterized by progressive motor dysfunction, dystonia, and memory loss, mimicking those observed in humans. We established a mouse model of dystonia by administering 3-NP. Given the reported neuroprotective effects of the endothelial growth factor angiopoietin-1 (Ang-1) and the anti-inflammatory integrin αvβ3 binding peptide C16, we performed this study to evaluate their combined effects on 3-NP striatal toxicity and their therapeutic potential with multiple methods using an in vivo mouse model. Sixty mice were equally and randomly divided into three groups: control, 3-NP treatment, and 3-NP+C16+Ang-1 treatment. Behavioral and electrophysiological tests were conducted and the effect of the combined C16+Ang-1 treatment on neural function recovery was determined. We found that C16+Ang-1 treatment alleviated 3-NP-induced behavioral, biochemical, and cellular alterations in the central nervous system and promoted function recovery by restoring vascular permeability and reducing inflammation in the micro-environment. In conclusion, our results confirmed the neuroprotective effect of combined C16+Ang-1 treatment and suggest their potential as a complementary therapeutic against 3-NP-induced dystonia.
- Subjects :
- Ang-1
Central Nervous System
Male
Aging
medicine.medical_treatment
Central nervous system
Excitotoxicity
Anti-Inflammatory Agents
dystonia model
Striatum
Endothelial Growth Factors
Pharmacology
medicine.disease_cause
Neuroprotection
Capillary Permeability
Random Allocation
improving micro-environment
Basal ganglia
medicine
Angiopoietin-1
Animals
C16
Neuroinflammation
Dystonia
Inflammation
Neurons
3-Nitropropionic acid
business.industry
Growth factor
Brain
Cell Biology
medicine.disease
Nitro Compounds
Corpus Striatum
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
Drug Therapy, Combination
Propionates
business
Peptides
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 19454589
- Volume :
- 13
- Issue :
- 14
- Database :
- OpenAIRE
- Journal :
- Aging (Albany NY)
- Accession number :
- edsair.doi.dedup.....dd73f16131f60f637da3be60e1ee601a