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Beta-asarone protects against MPTP-induced Parkinson's disease via regulating long non-coding RNA MALAT1 and inhibiting α-synuclein protein expression
Beta-asarone protects against MPTP-induced Parkinson's disease via regulating long non-coding RNA MALAT1 and inhibiting α-synuclein protein expression
- Source :
- Biomedicinepharmacotherapy = Biomedecinepharmacotherapie. 83
- Publication Year :
- 2016
-
Abstract
- Objective Numerous long non-coding RNAs (lncRNA) have been identified in neurodegenerative disorders including Parkinson’s disease (PD). Emerging evidence demonstrates that β-asarone functions as neuroprotective effects in both in vitro and in vivo models. However, the role of β-asarone and its potential mechanism in PD remain not completely clear. Methods MPTP-induced PD mouse model and SH-SY5Y cells subjected to MPP+ as its in vitro model were used to evaluate the effects of β-asarone on PD. LncRNA MALAT1 and α-synuclein expression were determined by real-time PCR and western blot methods. Results β-Asarone significantly increased the TH+ cells number and decreased the expression levels of MALAT1 and α-synuclein in midbrain tissue of PD mice. RNA pull-down and immunoprecipitation assays confirmed that MALAT1 associated with α-synuclein, leading to the increased stability of α-synuclein and its expression in SH-SY5Y cells. β-asarone elevated the viability of cells exposed to MPP+. Either overexpressed MALAT1 or α-synuclein could canceled the protective effect of β-asarone on cell viability. In PD mice, pcDNA-MALAT1 also decreased the TH+ cells number and increased the α-synuclein expression in PD mice with treatment of β-asarone. Conclusion β-Asarone functions as a neuroprotective effect in both in vivo and in vitro models of PD via regulating MALAT1 and α-synuclein expression.
- Subjects :
- 0301 basic medicine
Male
Pathology
medicine.medical_specialty
Tyrosine 3-Monooxygenase
Cell Survival
Allylbenzene Derivatives
Biology
Anisoles
Neuroprotection
03 medical and health sciences
chemistry.chemical_compound
Western blot
In vivo
Cell Line, Tumor
medicine
Animals
Humans
Viability assay
Pharmacology
Neurons
MALAT1
medicine.diagnostic_test
MPTP
RNA
Parkinson Disease
General Medicine
In vitro
Cell biology
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
Neuroprotective Agents
chemistry
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
alpha-Synuclein
RNA, Long Noncoding
Subjects
Details
- ISSN :
- 19506007
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Biomedicinepharmacotherapy = Biomedecinepharmacotherapie
- Accession number :
- edsair.doi.dedup.....ddae7347d06342e0f6d71ff4edf09a99