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Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation
- Source :
- International Journal of Molecular Sciences, Vol 21, Iss 5303, p 5303 (2020), International Journal of Molecular Sciences, Volume 21, Issue 15
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Chondroitin sulfate (CS) has antioxidative, anti-inflammatory, anti-osteoarthritic and hypoglycemic effects. However, whether it has antidiabetic osteoporosis effects has not been reported. Therefore, in this study, we established a STZ-induced diabetic rat model<br />CS (500 mg kg&minus<br />1 d&minus<br />1) was orally administrated for eight weeks to study its preventive effects on diabetic osteoporosis. The results showed that eight weeks of CS treatment improved the symptoms of diabetes<br />the CS-treated group has increased body weight, decreased water or food intake, decreased blood glucose, increased bone-mineral density, repaired bone morphology and decreased femoral osteoclasts and tibia adipocytes numbers. After CS treatment, bone histomorphometric parameters returned to normal, the levels of serum inflammatory cytokines (IL-1&beta<br />IL-6 and TNF-&alpha<br />) decreased significantly, serum SOD, GPX and CAT activities increased and MDA level increased. In the CS-treated group, the levels of serum ALP, CTX-1, TRACP 5b, osteocalcin and RANKL decreased and the serum RUNX 2 and OPG levels increased. Bone immunohistochemistry results showed that CS can effectively increase the expression of OPG and RUNX2 and reduce the expression of RANKL in diabetic rats. All of these indicate that CS could prevent STZ induced diabetic osteoporosis&mdash<br />mainly through decreasing blood glucose, antioxidative stress, anti-inflammation and regulation of OPG/RANKL expression. CS can therefore effectively prevent bone loss caused by diabetes.
- Subjects :
- Blood Glucose
0301 basic medicine
Osteoporosis
medicine.disease_cause
Rats, Sprague-Dawley
lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
oxidative stress
lcsh:QH301-705.5
Spectroscopy
chondroitin sulfate
biology
Chondroitin Sulfates
General Medicine
Computer Science Applications
RANKL
diabetic osteoporosis
030220 oncology & carcinogenesis
Osteocalcin
Immunohistochemistry
Female
bone-mineral density
musculoskeletal diseases
medicine.medical_specialty
Article
Catalysis
Diabetes Mellitus, Experimental
Proinflammatory cytokine
Diabetes Complications
Inorganic Chemistry
03 medical and health sciences
Internal medicine
Diabetes mellitus
medicine
Animals
Chondroitin sulfate
Physical and Theoretical Chemistry
Molecular Biology
Inflammation
business.industry
RANK Ligand
Organic Chemistry
Osteoprotegerin
medicine.disease
Rats
030104 developmental biology
Endocrinology
Gene Expression Regulation
lcsh:Biology (General)
lcsh:QD1-999
chemistry
biology.protein
business
Oxidative stress
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....ddb0a2ac9f4c009e51aa584f21c5a074
- Full Text :
- https://doi.org/10.3390/ijms21155303