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Expression profiling of G2/M phase regulatory proteins in normal, premalignant and malignant uterine cervix and their correlation with survival of patients
- Source :
- Journal of Cancer Research and Therapeutics. 6:167
- Publication Year :
- 2010
- Publisher :
- Medknow, 2010.
-
Abstract
- Cell regulatory G2/M phase proteins are the key regulators of mitosis and have been reported with abnormal expressions in various malignancies.To determine the expressions of these proteins in neoplastic uterine cervix tissue.This study evaluates the G2/M phase regulatory protein expression of Cyclin B1, Aurora-B, Pololike kinase 1 (PLK1) and LIM kinase1 (LIMK1) in tissues of 25 normal (control), 16 dysplastic (dysplasia) and 34 neoplastic (cancer) patients of uterine cervix. The expressions of different proteins were obtained by using Western Blot technique.One way analysis of variance (ANOVA), Pearson correlation, Kaplan-Meier and other tests are used for analysis.The level of expression of LIMK1 in cervical cancer patients was found to be significantly higher (P0.01) than both the controls and dysplasia. The expression of Aurora B and PLK1 in cervical cancer patients was also found to be significantly higher ( P0.05) than controls but it did not differ with dysplasia. However, the expression of Cyclin B1 was similar among cervical cancer patients, dysplasia and controls ( P0.05). The expression of all the above proteins showed significant ( P0.01) and inverse relation with the survival of cancer patients. Among the selected candidate proteins, it was LIMK1 that showed the most positive correlation with the aggressiveness of the disease and negative correlation (r= -0.64; P0.01) with the survival of patients.
- Subjects :
- Adult
G2 Phase
Pathology
medicine.medical_specialty
Blotting, Western
Uterine Cervical Neoplasms
Cell Cycle Proteins
Protein Serine-Threonine Kinases
Biology
Cervical intraepithelial neoplasia
Young Adult
Western blot
Aurora Kinases
Proto-Oncogene Proteins
medicine
Aurora Kinase B
Humans
Radiology, Nuclear Medicine and imaging
Cyclin B1
Survival rate
Aged
Neoplasm Staging
medicine.diagnostic_test
Gene Expression Profiling
Lim Kinases
Cancer
General Medicine
Middle Aged
Uterine Cervical Dysplasia
medicine.disease
Survival Rate
Gene expression profiling
Blot
Treatment Outcome
Oncology
Dysplasia
Case-Control Studies
Cancer research
Female
Precancerous Conditions
Cell Division
Subjects
Details
- ISSN :
- 09731482
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of Cancer Research and Therapeutics
- Accession number :
- edsair.doi.dedup.....ddea5d58d17df98a5c2789fdaca07970