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UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats
- Source :
- Molecular Medicine Reports.
- Publication Year :
- 2018
- Publisher :
- Spandidos Publications, 2018.
-
Abstract
- The present study investigated the potential role of UbiA prenyltransferase domain-containing 1 (UBIAD1) in the pathogenesis of hypertensive cardiac hypertrophy. Spontaneously hypertensive rats (SHRs) and Wistar‑Kyoto (WKY) rats at 8, 16 and 28 weeks of age were used. Blood pressure was measured using a non‑invasive tail cut‑off system. Cardiac functional index was assessed by arterial catheterization. Myocardial structure and cell apoptosis were evaluated by hematoxylin and eosin staining, and terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling assays, respectively. Myocardial expression of UBIAD1, coenzyme Q10 (CoQ10), endothelial nitric oxide synthase (eNOS) and atrial natriuretic peptide were evaluated by immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction. Circulating and myocardial expression of nitric oxide (NO) were measured using the Griess method. SHRs exhibited increased blood pressure and cardiomyocyte apoptosis, as well as cardiac hypertrophy, compared with age‑matched WKY rats. Myocardial expression of UBIAD1 was significantly decreased in SHRs in an age‑dependent manner. Similarly, myocardial CoQ10 and eNOS expression were significantly reduced in SHR compared to age‑matched WKY rats, and these expression levels additionally decreased further with aging. Serum and myocardial NO expression was additionally decreased in SHRs. Decreased UBIAD1 expression in SHR hearts was associated with decreased levels of CoQ10, eNOS and NO. Given the well‑established role of UBIAD1 in the regulation of NO signaling, reduced expression of UBIAD1 in SHR hearts potentially contributed to the pathogenesis of hypertensive cardiac hypertrophy. Therefore, UBIAD1 may represent a potential therapeutic target for clinical treatment of hypertensive cardiac hypertrophy.
- Subjects :
- Male
Cancer Research
medicine.medical_specialty
Nitric Oxide Synthase Type III
H&E stain
Cardiomegaly
Rats, Inbred WKY
Biochemistry
Nitric oxide
Pathogenesis
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Atrial natriuretic peptide
Enos
Rats, Inbred SHR
Internal medicine
Genetics
medicine
Animals
Molecular Biology
Coenzyme Q10
biology
business.industry
Dimethylallyltranstransferase
biology.organism_classification
Rats
Endocrinology
Blood pressure
Oncology
chemistry
Apoptosis
Hypertension
Molecular Medicine
030211 gastroenterology & hepatology
business
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....de0ed0883b30da9957d374f213796fd7