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Early highly active antiretroviral therapy enhances B-cell longevity: a 5 year follow up
- Source :
- The Pediatric infectious disease journal. 33(5)
- Publication Year :
- 2014
-
Abstract
- We have previously reported that an early initiation of highly active antiretroviral therapy (HAART) in HIV-1 vertically infected children enhanced the function of memory B-cells gained during childhood routine vaccinations. On the other hand, a significant waning of immunity was observed for patients with a late treatment. In this follow-up study, we report data from a sample of patients in our cohort including late-treated patients being revaccinated with routine childhood vaccines.The levels of serum antibodies and cellular immunity were measured by antigen-specific enzyme-linked immunosorbent assay and B-cell ELISpot. Moreover, flow cytometry on the frequencies of mature-activated (CD10-CD21-) and double-negative (CD27-IgD-) B-cells as hallmarks of immune activation and immune senescence, respectively, was performed for all patients.Reduced protective humoral immunity and cellular immunity to routine childhood vaccines was observed in late-treated patients. Moreover, we found that timing of HAART related with the frequencies of mature activated and double negative.Altogether the data presented in this follow-up study reenforce the importance for an early start of HAART in HIV-1 vertically infected individuals and suggest that timing of HAART is a fundamental factor to take into account for vaccination design in this population.
- Subjects :
- Microbiology (medical)
Male
Pediatrics
medicine.medical_specialty
Enzyme-Linked Immunospot Assay
5 year follow up
Time Factors
Adolescent
Cell Survival
media_common.quotation_subject
Enzyme-Linked Immunosorbent Assay
HIV Infections
Early initiation
Antibodies
Young Adult
Immunity
Antiretroviral Therapy, Highly Active
medicine
Humans
Young adult
Child
B cell
media_common
B-Lymphocytes
Vaccines
business.industry
Longevity
Flow Cytometry
Antiretroviral therapy
Vaccination
Infectious Diseases
medicine.anatomical_structure
Anti-Retroviral Agents
Pediatrics, Perinatology and Child Health
Immunology
Female
business
Follow-Up Studies
Subjects
Details
- ISSN :
- 15320987
- Volume :
- 33
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The Pediatric infectious disease journal
- Accession number :
- edsair.doi.dedup.....de46698ec4df7d367458af926f4c8fe3