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Syntaxin 17 promotes lipid droplet formation by regulating the distribution of acyl-CoA synthetase 3[S]
- Source :
- Journal of Lipid Research, Vol 59, Iss 5, Pp 805-819 (2018)
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- Lipid droplets (LDs) are ubiquitous organelles that contain neutral lipids and are surrounded by a phospholipid monolayer. How proteins specifically localize to the phospholipid monolayer of the LD surface has been a matter of extensive investigations. In the present study, we show that syntaxin 17 (Stx17), a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein whose expression in the liver is regulated by diet, participates in LD biogenesis by regulating the distribution of acyl-CoA synthetase (ACSL)3, a key enzyme for LD biogenesis that redistributes from the endoplasmic reticulum (ER) to LDs during LD formation. Stx17 interacts with ACSL3, but not with LD formation-unrelated ACSL1 or ACSL4, through its SNARE domain. The interaction occurs at the ER-mitochondria interface and depends on the active site occupancy of ACSL3. Depletion of Stx17 impairs ACSL3 redistribution to nascent LDs. The defect in LD maturation due to Stx17 knockdown can be compensated for by ACSL3 overexpression, suggesting that Stx17 increases the efficiency of ACSL3 redistribution to LDs. Moreover, we show that the interaction between Stx17 and ACSL3 during LD maturation may be regulated by synaptosomal-associated protein of 23 kDa.
- Subjects :
- 0301 basic medicine
Phospholipid
QD415-436
Syntaxin 17
ACSL4
Biochemistry
mitochondria-associated membrane
synaptosomal-associated protein of 23 kDa
Mice
03 medical and health sciences
chemistry.chemical_compound
Endocrinology
3T3-L1 Cells
Lipid droplet
Coenzyme A Ligases
Organelle
Animals
Humans
Receptor
Research Articles
Cells, Cultured
Qa-SNARE Proteins
Endoplasmic reticulum
soluble N-ethylmaleimide-sensitive factor attachment protein receptor
Hep G2 Cells
Lipid Droplets
Cell Biology
Cell biology
endoplasmic reticulum
HEK293 Cells
030104 developmental biology
chemistry
Female
triacylglycerol
Biogenesis
Subjects
Details
- Language :
- English
- ISSN :
- 00222275
- Volume :
- 59
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of Lipid Research
- Accession number :
- edsair.doi.dedup.....de789882cf973ada93fc165fa088afe4