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Composition of the bacterial biota in slime developed in two machines at a Canadian paper mill
- Source :
- Canadian Journal of Microbiology, Canadian Journal of Microbiology, NRC Research Press, 2011, 57 (2), pp.91-104. ⟨10.1139/w10-109⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- International audience; During the process of papermaking by pulp and paper plants, a thick and viscous deposits, termed slime, is quickly formed around the paper machines, which can affect the papermaking process. In this study, we explored the composition of the bacterial biota in slime that developed on shower pipes from 2 machines at a Canadian paper mill. Firstly, the composition was assessed for 12 months by DNA profiling with polymerase chain reaction coupled with denaturing gradient gel electrophoresis. Except for short periods (2-3 months), clustered analyses showed that the bacterial composition of the slime varied substantially over the year, with less than 50% similarity between the denaturing gradient gel electrophoresis profiles. Secondly, the screening of 16S rRNA gene libraries derived from 2 slime samples showed that the most abundant bacteria were related to 6 lineages, including Chloroflexi, candidate division OP10, Clostridiales, Bacillales, Burkholderiales, and the genus Deinococcus. Finally, the proportion of 8 bacterial lineages, such as Deinococcus sp., Meiothermus sp., and Chloroflexi, was determined by the Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization in 2 slime samples. The results showed a high proportion of Chloroflexi, Tepidimonas spp., and Schlegelella spp. in the slime samples.
- Subjects :
- MESH: Sequence Analysis, DNA
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Biofouling
Polymerase Chain Reaction
Applied Microbiology and Biotechnology
RNA, Ribosomal, 16S
MESH: DNA Fingerprinting
Candidate division
Deinococcus
MESH: In Situ Hybridization, Fluorescence
[INFO.INFO-BT]Computer Science [cs]/Biotechnology
MESH: Phylogeny
In Situ Hybridization, Fluorescence
Phylogeny
Meiothermus
0303 health sciences
biology
General Medicine
Biota
MESH: RNA, Ribosomal, 16S
Burkholderiales
MESH: Paper
Temperature gradient gel electrophoresis
DNA, Bacterial
Paper
Canada
Immunology
Industrial Waste
MESH: Biofilms
Microbiology
03 medical and health sciences
MESH: Biota
MESH: Canada
Botany
MESH: Gene Library
Genetics
MESH: Denaturing Gradient Gel Electrophoresis
MESH: Industrial Waste
Molecular Biology
Gene Library
030304 developmental biology
Bacteria
Tepidimonas
Denaturing Gradient Gel Electrophoresis
030306 microbiology
Clostridiales
fungi
MESH: Polymerase Chain Reaction
Sequence Analysis, DNA
MESH: Biofouling
biology.organism_classification
DNA Fingerprinting
MESH: DNA, Bacterial
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Bacteria
Biofilms
bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 00084166 and 14803275
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology, Canadian Journal of Microbiology, NRC Research Press, 2011, 57 (2), pp.91-104. ⟨10.1139/w10-109⟩
- Accession number :
- edsair.doi.dedup.....de9c4bd1f3c995b6ac5ffb1659a45441