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Analysis of tissue-specific methylation patterns of mouse mammary tumor virus DNA by two-dimensional Southern blotting
- Source :
- Journal of Virology. 54:726-730
- Publication Year :
- 1985
- Publisher :
- American Society for Microbiology, 1985.
-
Abstract
- We used a two-dimensional Southern blotting procedure to analyze the tissue-specific methylation patterns of the five endogenous mouse mammary tumor viruses in the GR/A mouse strain. Our findings suggest that in certain tissues (brain, kidney, and liver) all proviruses are extensively methylated. In other tissues (spleen, placenta, and testes) all proviruses are hypomethylated to some degree. In these tissues individual proviruses display both quantitative and qualitative differences in methylation. We interpret the general patterns of tissue-specific hypomethylation in terms of a "hitch-hiker" model: mouse mammary tumor virus proviral methylation patterns reflect the tissue-specific activity of neighboring sequences. The observation that certain sites on particular proviruses are differentially methylated in a tissue-specific fashion may reflect tissue-specific differences in the makeup or conformation, or both, of proviral-containing chromatin.
- Subjects :
- Male
viruses
Placenta
Immunology
Endogeny
Methylation
Microbiology
Mice
chemistry.chemical_compound
Pregnancy
Virology
medicine
Animals
Southern blot
Electrophoresis, Agar Gel
Mammary tumor
biology
Mouse mammary tumor virus
biology.organism_classification
Molecular biology
Chromatin
medicine.anatomical_structure
Mammary Tumor Virus, Mouse
chemistry
Organ Specificity
Insect Science
DNA, Viral
Female
Spleen
DNA
Research Article
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....deebabe7f019ecd1a7617fde03e4a5d2