Back to Search
Start Over
Molecular characterization of mudskipper (Boleophthalmus pectinirostris) hypoxia-inducible factor-1α (HIF-1α) and analysis of its function in monocytes/macrophages
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 5, p e0177960 (2017)
- Publication Year :
- 2016
-
Abstract
- Hypoxia-inducible factor-1α (HIF-1α) plays a critical role in immune and inflammatory responses and is important in controlling a variety of processes in monocytes and macrophages. However, very little information is available about the functions of HIF-1α in fish monocytes/macrophages (MO/MФ). In this study, the cDNA sequence of the mudskipper (Boleophthalmus pectinirostris) HIF-1α gene (BpHIF-1α) was determined. Sequence comparison and phylogenetic tree analysis showed that BpHIF-1α is clustered in the fish HIF-1α tree. Constitutive expression of BpHIF-1α mRNA was detected by real-time quantitative PCR in all tested tissues, and the expression was found to be dramatically increased in the skin, liver, spleen, and kidney after Edwardsiella tarda infection. In addition, hypoxia and infection induced the expression of the BpHIF-1α transcript and protein in MO/MФ, respectively. Hypoxia caused an increase in phagocytic and bactericidal capacity of mudskipper MO/MФ in a BpHIF-1α-dependent manner. BpHIF-1α induced an anti-inflammatory status in MO/MФ upon E. tarda infection and hypoxia. Therefore, BpHIF-1α may play a predominant role in the modulation of mudskipper MO/MФ function in the innate immune system.
- Subjects :
- 0301 basic medicine
lcsh:Medicine
Biochemistry
Monocytes
White Blood Cells
Animal Cells
Gene expression
Medicine and Health Sciences
Small interfering RNAs
Cloning, Molecular
RNA, Small Interfering
lcsh:Science
Hypoxia
Edwardsiella tarda
Cells, Cultured
Skin
Multidisciplinary
Messenger RNA
Enterobacteriaceae Infections
Fishes
04 agricultural and veterinary sciences
Cell Hypoxia
Nucleic acids
medicine.anatomical_structure
Infectious Diseases
Hypoxia-inducible factors
Liver
Cell Processes
RNA Interference
Cellular Types
Anatomy
Research Article
Skin Infections
Phagocytosis
Immune Cells
Immunology
Spleen
Dermatology
Biology
03 medical and health sciences
Immune system
Immunity
medicine
Genetics
Animals
Amino Acid Sequence
Non-coding RNA
Inflammation
Innate immune system
Blood Cells
Base Sequence
Macrophages
lcsh:R
Biology and Life Sciences
Sequence Analysis, DNA
Cell Biology
biology.organism_classification
Hypoxia-Inducible Factor 1, alpha Subunit
Molecular biology
Immunity, Innate
Gene regulation
Gastrointestinal Tract
030104 developmental biology
040102 fisheries
0401 agriculture, forestry, and fisheries
RNA
lcsh:Q
Sequence Alignment
Digestive System
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....def5f798323e491710c4fa3d028c75a7