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The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs
- Source :
- Viruses, Vol 12, Iss 2, p 170 (2020), Viruses, Volume 12, Issue 2
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Papillomavirus (PV) mainly infects the squamous epithelium and may potentially lead to benign or even malignant cutaneous lesions. However, the malignant transforming ability has been identified in several types of PVs. In humans, papillomavirus (HPV) type 16 and 18 are the most prevalent causative agents of cervical cancer. Therefore, vaccines are being developed to protect against these types. For dogs, there have been limited investigations into the association of different canine papillomavirus (CPV) genotypes with malignant lesions. Understanding the high-risk CPV genotype(s) responsible for these malignant lesions would contribute to the development of interventions for preventing CPV-induced carcinomas. In the present study, a retrospective cohort of 102 pathologically confirmed papillomas and 212 squamous cell carcinomas (SCCs) were included. The viral genome and antigens in the formalin-fixed paraffin-embedded (FFPE) tissues were detected using PCR targeting pan PV E1 and COPV L1 genes and by immunohistochemistry staining (IHC), respectively. PVs were successfully detected from 11 FFPE cutaneous tissues and four oral tissues using pan PV E1- and COPV L1-based PCR, respectively. After sequencing, CPV 1, CPV 2, and CPV 6 were detected in the benign lesions using PCR and were confirmed through IHC. While CPV 9 and CPV 15 were first detected in the SCCs of dogs, CPV 16 was most often detected in SCC specimens. The association and confirmative demonstration of viral genes and intralesional antigens of CPV 9, CPV 15, and CPV 16 in SCCs highlight the potential risk of these genotypes of CPVs in malignant transformation.
- Subjects :
- 0301 basic medicine
squamous cell carcinoma
Pathology
medicine.medical_specialty
Skin Neoplasms
040301 veterinary sciences
animal diseases
viruses
lcsh:QR1-502
Article
canine oral papillomavirus
lcsh:Microbiology
Malignant transformation
0403 veterinary science
03 medical and health sciences
Dogs
Antigen
Virology
Genotype
Medicine
Animals
Dog Diseases
Papillomaviridae
Retrospective Studies
Skin
Cervical cancer
Paraffin Embedding
business.industry
canine papillomavirus
Papillomavirus Infections
04 agricultural and veterinary sciences
medicine.disease
Immunohistochemistry
Epithelium
Staining
papilloma
030104 developmental biology
Infectious Diseases
medicine.anatomical_structure
Cell Transformation, Neoplastic
DNA, Viral
Carcinoma, Squamous Cell
Papilloma
business
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Volume :
- 12
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....df0b83c8c62ca0cfcf9786f7c24d1f0d