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Transcriptome analyses reveal the utilization of nitrogen sources and related metabolic mechanisms of Sporosarcina pasteurii
- Source :
- PLoS ONE, Vol 16, Iss 2, p e0246818 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- In recent years, Sporosarcina pasteurii (S. pasteurii) has become one of the most popular bacteria in microbially induced calcium carbonate precipitation (MICP). Various applications have been developed based on the efficient urease that can induce the precipitation of calcium carbonate. However, the metabolic mechanism related to biomineralization of S. pasteurii has not been clearly elucidated. The process of bacterial culture and biomineralization consumes a large amount of urea or ammonium salts, which are usually used as agricultural fertilizers, not to mention probable environmental pollutions caused by the excessive use of these raw materials. Therefore, it is urgent to reveal the mechanism of nitrogen utilization and metabolism of S. pasteurii. In this paper, we compared the growth and gene expression of S. pasteurii under three different culture conditions through transcriptome analyses. GO and KEGG analyses revealed that both ammonium and urea were direct nitrogen sources of S. pasteurii, and the bacteria could not grow normally in the absence of ammonium or urea. To the best of our knowledge, this paper is the first one to reveal the nitrogen utilization mechanism of S. pasteurii through transcriptome methods. Furthermore, the presence of ammonium might promote the synthesis of intracellular ATP and enhance the motility of the bacteria. There should be an ATP synthesis mechanism associated with urea hydrolysis catalyzed by urease in S. pasteurii.
- Subjects :
- Urease
Gene Expression
Pathology and Laboratory Medicine
Biochemistry
chemistry.chemical_compound
Adenosine Triphosphate
Cell Wall
Ammonium Compounds
Medicine and Health Sciences
Urea
Multidisciplinary
biology
Organic Compounds
Chemistry
Hydrolysis
Chemical Reactions
Genomics
Sporosarcina pasteurii
Enzymes
Up-Regulation
Flagella
Physical Sciences
Ureases
Medicine
Metabolic Pathways
Cellular Structures and Organelles
Pathogens
Transcriptome Analysis
Research Article
Pathogen Motility
Sporosarcina
Virulence Factors
Nitrogen
Science
DNA transcription
Down-Regulation
Genetics
Ammonium
Nitrogen cycle
Gene Expression Profiling
Organic Chemistry
Chemical Compounds
Biology and Life Sciences
Proteins
Computational Biology
Cell Biology
Gene Expression Regulation, Bacterial
Ammonia volatilization from urea
Genome Analysis
biology.organism_classification
Biosynthetic Pathways
Metabolism
Gene Ontology
Genes, Bacterial
Enzymology
biology.protein
Transcriptome
Bacteria
Biomineralization
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....df318d20057a5f835bdcd1af777a5b8d