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Cytokine-Induced CEACAM1 Expression on Keratinocytes Is Characteristic for Psoriatic Skin and Contributes to a Prolonged Lifespan of Neutrophils
- Source :
- Journal of Investigative Dermatology. 129(3):671-681
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) is a cell-surface glycoprotein, belonging to the carcinoembryonic antigen family, expressed by human neutrophils, epithelial cells, activated T and NK cells. CEACAM1 is expressed as a cell-surface molecule with different isoforms or can be secreted as a soluble protein. Here, we show that keratinocytes in the outer epidermal layer of psoriatic skin express CEACAM1, unlike those in healthy skin or in cutaneous lesions of patients with atopic or nummular dermatitis. Stimulation of primary human keratinocytes or in vitro reconstituted epidermis with culture supernatants of activated psoriatic lesion-infiltrating T cells, IFN-gamma or oncostatin M, but not IL-17, induced the expression of transcripts for the CEACAM1-long and -short isoforms and cell-surface CEACAM1, whereas soluble CEACAM1 was not produced. The uppermost layers of the epidermis in psoriatic lesions also contain neutrophils, a cell type with inflammatory and antimicrobial properties. Coculture of CEACAM1-expressing keratinocytes or CHO transfectants with neutrophils delayed spontaneous apoptosis of the latter cells. These results show that cytokine-induced cell-surface expression of CEACAM1 by keratinocytes in the context of a psoriatic environment might contribute to the persistence of neutrophils and thus to ongoing inflammation and the decreased propensity for skin infection, typical for patients with psoriasis.
- Subjects :
- Keratinocytes
Cell type
Neutrophils
medicine.medical_treatment
T-Lymphocytes
Inflammation
Oncostatin M
Dermatology
Biology
Biochemistry
Interferon-gamma
Mice
Antigen
Antigens, CD
Psoriasis
medicine
Animals
Humans
Molecular Biology
Skin
Mice, Inbred BALB C
Epidermis (botany)
Interleukin-17
Cell Biology
medicine.disease
Molecular biology
Carcinoembryonic Antigen
medicine.anatomical_structure
Cytokine
Immunology
biology.protein
Female
medicine.symptom
Keratinocyte
Cell Adhesion Molecules
Subjects
Details
- ISSN :
- 0022202X
- Volume :
- 129
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Investigative Dermatology
- Accession number :
- edsair.doi.dedup.....df3d3a922064e3041cc344947df855bb
- Full Text :
- https://doi.org/10.1038/jid.2008.303