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Two Endosomal NHX-Type Na+/H+ Antiporters are Involved in Auxin-Mediated Development in Arabidopsis thaliana
- Source :
- PLANT AND CELL PHYSIOLOGY
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- In Arabidopsis thaliana, the endosomal-localized Na+/H+ antiporters NHX5 and NHX6 regulate ion and pH homeostasis and are important for plant growth and development. However, the mechanism by which these endosomal NHXs function in plant development is not well understood. Auxin modulates plant growth and development through the formation of concentration gradients in plant tissue to control cell division and expansion. Here, we identified a role for NHX5 and NHX6 in the establishment and maintenance of auxin gradients in embryo and root tissues. We observed developmental impairment and abnormal cell division in embryo and root tissues in the double knockout nhx5 nhx6, consistent with these tissues showing high expression of NHX5 and NHX6. Through confocal microscopy imaging with the DR5:: GFP auxin reporter, we identify defects in the perception, accumulation and redistribution of auxin in nhx5 nhx6 cells. Furthermore, we find that the steady-state levels of the PIN-FORMED (PIN) auxin efflux carriers PIN1 and PIN2 are reduced in nhx5 nhx6 root cells. Our results demonstrate that NHX5 and NHX6 function in auxin-mediated plant development by maintaining PIN abundance at the plasma membrane, and provide new insight into the regulation of plant development by endosomal NHX antiporters.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Auxin efflux
Technology and Engineering
Sodium-Hydrogen Exchangers
GNOM ARF-GEF
Arabidopsis thaliana
Physiology
Endosome
CELL POLARITY
CARRIER
Arabidopsis
Endosomes
Plant Science
01 natural sciences
PROTEIN TRAFFICKING
Green fluorescent protein
Lateral root
PIN2
03 medical and health sciences
Gene Expression Regulation, Plant
Auxin
NHX5
Cell polarity
NHX6
chemistry.chemical_classification
Indoleacetic Acids
Arabidopsis Proteins
fungi
Biology and Life Sciences
PERMEASE AUX1
PIN
food and beverages
LOCALIZATION
Cell Biology
General Medicine
Antiporters
TRANSPORT
3. Good health
Cell biology
Chemistry
030104 developmental biology
chemistry
PLANT-GROWTH
EMERGING ROLES
PIN1
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14719053 and 00320781
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Plant and Cell Physiology
- Accession number :
- edsair.doi.dedup.....df3eaec497838411ca1a6f29ee14f424
- Full Text :
- https://doi.org/10.1093/pcp/pcy090