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The more the merrier: high-throughput single-molecule techniques
- Source :
- Biochemical Society Transactions. 45:759-769
- Publication Year :
- 2017
- Publisher :
- Portland Press Ltd., 2017.
-
Abstract
- The single-molecule approach seeks to understand molecular mechanisms by observing biomolecular processes at the level of individual molecules. These methods have led to a developing understanding that for many processes, a diversity of behaviours will be observed, representing a multitude of pathways. This realisation necessitates that an adequate number of observations are recorded to fully characterise this diversity. The requirement for large numbers of observations to adequately sample distributions, subpopulations, and rare events presents a significant challenge for single-molecule techniques, which by their nature do not typically provide very high throughput. This review will discuss many developing techniques which address this issue by combining nanolithographic approaches, such as zero-mode waveguides and DNA curtains, with single-molecule fluorescence microscopy, and by drastically increasing throughput of force-based approaches such as magnetic tweezers and laminar-flow techniques. These methods not only allow the collection of large volumes of single-molecule data in single experiments, but have also made improvements to ease-of-use, accessibility, and automation of data analysis.
- Subjects :
- 0301 basic medicine
Magnetic tweezers
DNA CURTAINS
Computer science
Distributed computing
IMAGING REVEALS
Biophysics
LAGGING-STRAND SYNTHESIS
Nanotechnology
Biochemistry
NANOSCALE CURTAIN RODS
MODE WAVE-GUIDES
03 medical and health sciences
NUCLEIC ACID INTERACTIONS
Rare events
ACOUSTIC FORCE SPECTROSCOPY
Throughput (business)
business.industry
REVEALS MECHANISMS
Proteins
DNA
Sequence Analysis, DNA
Automation
MAGNETIC TWEEZERS
030104 developmental biology
INTERNAL-REFLECTION FLUORESCENCE
Microscopy, Fluorescence
business
Subjects
Details
- ISSN :
- 14708752 and 03005127
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Biochemical Society Transactions
- Accession number :
- edsair.doi.dedup.....df5e584602f67ad411050568d4107244
- Full Text :
- https://doi.org/10.1042/bst20160137