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Enumeration and Molecular Characterisation of Circulating Tumour Cells in Endometrial Cancer
- Source :
- Oncology. 91:48-54
- Publication Year :
- 2016
- Publisher :
- S. Karger AG, 2016.
-
Abstract
- Background: This is a feasibility study to determine whether circulating tumour cells (CTCs) are detectable and suitable for molecular profiling in advanced endometrial cancer (aEC). Method: Between October 2012 and February 2014, 30 patients with aEC had baseline and up to 3 follow-up samples. CTCs and stathmin expression were evaluated using the CellSearch platform. Epithelial cell adhesion molecule (EpCAM) and stathmin immunohistochemistry were performed on FFPE tumour tissue. Results: Eighteen from 30 (60%) patients had detectable CTCs during study [1 CTC (n = 7), 2 (n = 4), 3 (n = 1), 4 (n = 2), 7 (n = 1), 8 (n = 1), 22 (n = 1), 172 (n = 1) in 7.5 ml blood]. Ten from 18 patients had between 50 and 100% of detectable CTCs that were stathmin positive. More CTC-positive than CTC-negative patients had non-endometrioid versus endometrioid histology, tumour size ≥5 versus 0.05, 95% confidence interval 0.7-16.2]. Twenty-one tumour blocks were tested for EpCAM and stathmin immunohistochemistry (IHC). Stathmin tumour immunostaining scores (TIS) on IHC were higher in CTC-positive patients. Conclusion: CTC enumeration and molecular profiling with stathmin on the CellSearch platform is feasible in aEC. Stathmin TIS on IHC, a known prognostic marker in EC, was associated with CTC positivity.
- Subjects :
- 0301 basic medicine
Oncology
Cancer Research
medicine.medical_specialty
Colorectal cancer
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Breast cancer
Internal medicine
medicine
Enumeration
Carcinoma
Humans
Aged
Neoplasm Staging
Aged, 80 and over
business.industry
Endometrial cancer
Epithelial cell adhesion molecule
General Medicine
Middle Aged
Epithelial Cell Adhesion Molecule
Neoplastic Cells, Circulating
medicine.disease
Immunohistochemistry
Endometrial Neoplasms
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Cancer research
Stathmin
Female
Ovarian cancer
business
Carcinoma, Endometrioid
Subjects
Details
- ISSN :
- 14230232 and 00302414
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Oncology
- Accession number :
- edsair.doi.dedup.....df8161f5cd7e2baef87c52a1172f9ad8