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Cutting Edge: Dendritic Cell Actin Cytoskeletal Polarization during Immunological Synapse Formation Is Highly Antigen-Dependent
- Source :
- The Journal of Immunology. 171:4479-4483
- Publication Year :
- 2003
- Publisher :
- The American Association of Immunologists, 2003.
-
Abstract
- Dendritic cells (DC) actively rearrange their actin cytoskeleton to participate in formation of the immunological synapse (IS). In this study, we evaluated the requirements for DC participation in the IS. DC rearrange their actin cytoskeleton toward naive CD4+ T cells only in the presence of specific MHC-peptide complexes. In contrast, naive CD4+ T cells polarized their cytoskeletal proteins in the absence of Ag. DC cytoskeletal rearrangement occurred at the same threshold of peptide-MHC complexes as that required for T cell activation. Furthermore, T cell activation was inhibited by specific blockade of DC cytoskeletal rearrangement. When TCR-MHC interaction was bypassed by using Con A-activated T cells, DC polarization was abrogated. In addition, directional ligation of MHC class II resulted in DC cytoskeletal polarization. Our findings suggest that a high Ag specificity is required for DC IS formation and that MHC class II signaling plays a central role in this process.
- Subjects :
- T cell
Molecular Sequence Data
Immunology
Mice, Transgenic
Cell Communication
Ligands
Lymphocyte Activation
Immunological synapse
Mice
T-Lymphocyte Subsets
medicine
Animals
Immunology and Allergy
Amino Acid Sequence
Antigens
Cytoskeleton
Cells, Cultured
Actin
Cell Aggregation
Mice, Inbred BALB C
MHC class II
biology
Immunological synapse formation
Histocompatibility Antigens Class II
Dendritic Cells
Dendritic cell
Actin cytoskeleton
Actins
Coculture Techniques
Cell biology
Mice, Inbred C57BL
medicine.anatomical_structure
biology.protein
Peptides
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 171
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....df8a65a6b2b2b9b69d450c3da2ed6229