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Anti-Ro52 reactivity is an independent and additional serum marker in connective tissue disease
- Publication Year :
- 2002
-
Abstract
- Objective: To determine whether anti-Ro52 is an independent serum marker in connective tissue disease. Methods: Over a two year period, 1727 consecutive antinuclear antibody (ANA) positive serum samples were analysed in parallel by double immunodiffusion with thymus/spleen nuclear extract and by line immunoassay with recombinant Ro52, recombinant La/SSB, and natural Ro60. Sera that were only reactive towards Ro52 were further analysed by a variety of additional anti-SSA/Ro detection methods and by specific anti-Ro52 and anti-Ro60 assays. Natural purified SSA/Ro was analysed by immunoblot and protein sequencing. Results: Analysis of natural purified SSA/Ro (Immunovision, Springdale, AR) showed only Ro60 and no immunoreactive Ro52. Consequently, assays based on this substrate only identify sera with anti-Ro60 reactivity. Twenty serum samples showed anti-Ro52 without anti-Ro60 and anti-SSB/La on line immunoassay. By additional testing, 2/20 sera were found positive for anti-Ro60 reactivity. The remaining 18 sera were not identified by any of the classical anti-SSA/Ro assays and were considered to be reactive only with Ro52 and not with Ro60. This anti-Ro52 reactivity was confirmed by natural and recombinant Ro52 in 16/18 cases. 12/18 sera corresponded to connective tissue diseases. Conclusion: Anti-Ro52 positive sera without any evidence of anti-Ro60 and anti-La/SSB reactivity can be considered as an independent group that is systematically missed by classical anti-SSA/Ro detection methods owing to a bias towards anti-Ro60 reactivity. The anti-Ro52 sera are precipitin negative, not retrieved by SSA/Ro enzyme linked immunosorbent assays (ELISAs) based on natural SSA/Ro, and show no specific ANA fluorescence staining pattern. These findings together with the clinical data indicate that anti-Ro52 should be considered as an additional and independent serum marker.
- Subjects :
- musculoskeletal diseases
Systemic disease
Anti-nuclear antibody
Concise Report
Immunology
Connective tissue
Enzyme-Linked Immunosorbent Assay
Cross Reactions
Autoantigens
General Biochemistry, Genetics and Molecular Biology
law.invention
Rheumatology
law
RNA, Small Cytoplasmic
medicine
Immunology and Allergy
Humans
Connective Tissue Diseases
Fluorescent Antibody Technique, Indirect
biology
medicine.disease
Ouchterlony double immunodiffusion
Precipitin
Connective tissue disease
Molecular biology
Recombinant Proteins
stomatognathic diseases
medicine.anatomical_structure
Ribonucleoproteins
Antibodies, Antinuclear
Recombinant DNA
biology.protein
Antibody
Biomarkers
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....e02100a8e4fad04a95236df4b79de405