Back to Search
Start Over
Neutrophil Extracellular Traps Are Markers of Wound Healing Impairment in Patients with Diabetic Foot Ulcers Treated in a Multidisciplinary Setting
- Publication Year :
- 2019
- Publisher :
- Mary Ann Liebert, Inc., publishers, 2019.
-
Abstract
- Objective: Neutrophil extracellular traps (NETs) are associated with impaired wound healing in diabetes. This study evaluates the association between NET-specific markers and wound healing among diabetic foot ulcer (DFU) patients treated in a multidisciplinary setting. Approach: Clinical data of diabetic patients with active foot ulcers who presented to our team between January 1, 2016 and June 30, 2017 were recorded. The diabetic ulcer severity score (DUSS) and wound, ischemia, and foot infection (WIfI) score were calculated. NET-specific markers in plasma and wound tissues were tested. The capacity for plasma and platelets to prime neutrophils to release NETs was assessed. The prognostic value of NET-specific markers for wound healing was evaluated. Results: NET-specific markers were significantly higher in DFU patients than in diabetic patients without DFU or healthy controls and were found to correlate positively with DUSS or WIfI score. Elastase levels in ulcer tissue significantly increased in wounds with infections and delayed healing. Higher levels of NET release were observed after the stimulation of plasma or platelets from ulcer-related vessels than from nonulcer-related vessels of the DFU patients. Citrullinated histone 3 (citH3) was identified as a risk factor for wound healing impairment and amputation. The patients with the highest quartile of citH3 levels presented significantly lower healing rates and higher amputation rates than those with the lower three quartiles. Innovation: This study extended current knowledge of NETs on wound healing in DFU patients. Conclusion: NET-specific markers negatively correlated with wound healing in DFU patients, and citH3 is a potential marker.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
medicine.medical_treatment
Ischemia
Interdisciplinary Studies
Critical Care and Intensive Care Medicine
Gastroenterology
Extracellular Traps
Severity of Illness Index
Amputation, Surgical
Anti-Citrullinated Protein Antibodies
Histones
030207 dermatology & venereal diseases
03 medical and health sciences
0302 clinical medicine
Risk Factors
Internal medicine
Diabetes mellitus
medicine
Humans
Risk factor
Discovery Express
Foot Ulcer
Aged
Retrospective Studies
Aged, 80 and over
Patient Care Team
Wound Healing
business.industry
Neutrophil extracellular traps
Middle Aged
medicine.disease
Prognosis
Diabetic foot
Diabetic Foot
030104 developmental biology
Diabetic foot ulcer
Amputation
Emergency Medicine
Female
business
Wound healing
Biomarkers
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....e075f0f3bcb5f06c677c62f505f389aa