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Contribution of Palmitic Acid to Epidermal Morphogenesis and Lipid Barrier Formation in Human Skin Equivalents
- Source :
- International Journal of Molecular Sciences, 20(23), 6069, 'International Journal of Molecular Sciences ', vol: 20, pages: 6069-1-6069-18 (2019), International Journal of Molecular Sciences, Volume 20, Issue 23, International journal of molecular sciences, 20(23). Multidisciplinary Digital Publishing Institute (MDPI)
- Publication Year :
- 2019
-
Abstract
- The outermost barrier layer of the skin is the stratum corneum (SC), which consists of corneocytes embedded in a lipid matrix. Biosynthesis of barrier lipids occurs de novo in the epidermis or is performed with externally derived lipids. Hence, in vitro developed human skin equivalents (HSEs) are developed with culture medium that is supplemented with free fatty acids (FFAs). Nevertheless, the lipid barrier formation in HSEs remains altered compared to native human skin (NHS). The aim of this study is to decipher the role of medium supplemented saturated FFA palmitic acid (PA) on morphogenesis and lipid barrier formation in HSEs. Therefore, HSEs were developed with 100% (25 &mu<br />M), 10%, or 1% PA. In HSEs supplemented with reduced PA level, the early differentiation was delayed and epidermal activation was increased. Nevertheless, a similar SC lipid composition in all HSEs was detected. Additionally, the lipid organization was comparable for lamellar and lateral organization, irrespective of PA concentration. As compared to NHS, the level of monounsaturated lipids was increased and the FFA to ceramide ratio was drastically reduced in HSEs. This study describes the crucial role of PA in epidermal morphogenesis and elucidates the role of PA in lipid barrier formation of HSEs.
- Subjects :
- 0301 basic medicine
Keratinocytes
Human skin
Fatty Acids, Nonesterified
Palmitic acid
030207 dermatology & venereal diseases
chemistry.chemical_compound
0302 clinical medicine
lipid metabolism
Morphogenesis
palmitic acid
Spectroscopy
Cells, Cultured
artificial skin
Skin
mass spectrometry
integumentary system
Chemistry
Cell Differentiation
General Medicine
Lipids
Computer Science Applications
Cell biology
medicine.anatomical_structure
lipids (amino acids, peptides, and proteins)
free fatty acid
Ceramide
Ceramides
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Biosynthesis
Stratum corneum
medicine
Humans
ceramide
Physical and Theoretical Chemistry
Molecular Biology
Skin, Artificial
Corneocyte
bioengineering
Epidermis (botany)
Lipogenesis
Organic Chemistry
Lipid metabolism
030104 developmental biology
Epidermal Cells
Epidermis
primary cell culture
Subjects
Details
- Language :
- English
- ISSN :
- 14220067 and 16616596
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences, 20(23), 6069, 'International Journal of Molecular Sciences ', vol: 20, pages: 6069-1-6069-18 (2019), International Journal of Molecular Sciences, Volume 20, Issue 23, International journal of molecular sciences, 20(23). Multidisciplinary Digital Publishing Institute (MDPI)
- Accession number :
- edsair.doi.dedup.....e0849a53d852331e372cb894dcf0b796