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Different binding of stimulatory-type and blocking-type TSH receptor antibody with guinea-pig testis membrane
- Source :
- Acta endocrinologica. 125(5)
- Publication Year :
- 1991
-
Abstract
- A receptor assay using [125I]bTSH-binding to guinea-pig testis membrane was developed. Unlabelled hCG and FSH inhibited [125I]bTSH binding. In patients with Graves' disease and in untreated hyperthyroid patients, almost all long-acting thyroid stimulators and thyroid-stimulating antibodies, respectively did not inhibit [125I]bTSH binding, which on the other hand was inhibited by thyroid stimulation blocking antibodies in patients with primary hypothyroidism. When the inhibitory effect on the binding of [125I]hCG and 125I-synthetic α-subunit peptide (α26-46) of hCG to testis membrane was examined, bTSH resulted in a significant inhibition. However, all three kinds of TSH receptor antibodies had no inhibitory effect. This study demonstrated 1. interaction of α-subunit of TSH and hCG with the testicular receptor; 2. binding of thyroid stimulation-blocking antibody and lack of binding of thyroid-stimulating antibody to the testicular TSH receptor in spite of binding of these TSH receptor antibodies to the thyroidal TSH receptor, and 3. lack of binding of thyroid-stimulating antibody and thyroid stimulation-blocking antibody to the testicular gonadotropin receptor.
- Subjects :
- Male
endocrine system
medicine.medical_specialty
endocrine system diseases
Endocrinology, Diabetes and Metabolism
Guinea Pigs
Molecular Sequence Data
Thyroid Gland
Thyrotropin
Biology
Chorionic Gonadotropin
Antibodies
Iodine Radioisotopes
Radioligand Assay
Endocrinology
Receptors, Gonadotropin
Internal medicine
Blocking antibody
Testis
medicine
Animals
Amino Acid Sequence
Receptor
Autoantibodies
Thyroid
Cell Membrane
Receptors, Thyrotropin
General Medicine
Testicular receptor
medicine.anatomical_structure
biology.protein
Thyroid Stimulating Immunoglobulin
Long-Acting Thyroid Stimulator
Gonadotropin receptor
Antibody
Follicle Stimulating Hormone
hormones, hormone substitutes, and hormone antagonists
Immunoglobulins, Thyroid-Stimulating
Protein Binding
Subjects
Details
- ISSN :
- 00015598
- Volume :
- 125
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Acta endocrinologica
- Accession number :
- edsair.doi.dedup.....e084f2b70ffd459c0124de5b926e4294